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Evaluation of loss of function as an explanation for SPG4-based hereditary spastic paraplegia.
Solowska JM, Garbern JY, Baas PW
Hum Mol Genet. 2010 Jul 15;19(14):2767-79. doi: 10.1093/hmg/ddq177. Epub 2010 Apr 29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
87715pCMV-Tag 3A/myc-M1E112KExpresses myc-M1E112K spastin isoform in mammalian cells
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87716pCMV-Tag 3A/myc-M1L195VExpresses myc-M1L195V spastin isoform in mammalian cells
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87717pCMV-Tag 3A/myc-M1S44LExpresses myc-M1S44L spastin isoform in mammalian cells
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87718pCMV-Tag 3A/myc-M1S46XExpresses myc-M1S46X spastin isoform in mammalian cells
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87719pCMV-Tag 3A/WT myc-M1Expresses WT myc-M1 spastin isoform in mammalian cells
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87720pCMV-Tag 3B/myc-M87E112KExpresses myc-M87E112K spastin isoform in mammalian cells
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87721pCMV-Tag 3B/myc-M87L195VExpresses myc-M87L195V spastin isoform in mammalian cells
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87722pCMV-Tag 3B/WT myc-M87Expresses WT myc-M87 spastin isoform in mammalian cells
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87723pCMV-Tag/E112K with 5'UTRExpresses E112K spastin M1 and M87 in mammalian cells
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87724pCMV-Tag/E112K without 5'UTRExpresses E112K spastin M1 and M87 in mammalian cells
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87725pCMV-Tag/L195V with 5'UTRExpresses L195V spastin M1 and M87 in mammalian cells
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87726pCMV-Tag/L195V without 5'UTRExpresses L195V spastin M1 and M87 in mammalian cells
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87727pCMV-Tag/S44L with 5'UTRExpresses S44L spastin M1 and M87 in mammalian cells
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87728pCMV-Tag/S44L without 5'UTRExpresses S44L spastin M1 and M87 in mammalian cells
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87729pCMV-Tag/S46X with 5'UTRExpresses S46X spastin M1 and M87 in mammalian cells
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87730pCMV-Tag/S46X without 5'UTRExpresses S46X spastin M1 and M87 in mammalian cells
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87731pCMV-Tag/WT SPAST with 5'UTRExpresses WT spastin M1 and M87 in mammalian cells
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87732pCMV-Tag/WT SPAST without 5'UTRExpresses WT spastin M1 and M87 in mammalian cells
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