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Permanent genetic memory with >1-byte capacity.
Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu TK, Voigt CA
Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60563pReporter_2A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60564pReporter_3A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60565pReporter_4A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60566pReporter_5A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60567pReporter_7A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60568pReporter_8A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60569pReporter_9A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60570pReporter_10A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60571pReporter_11A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60572pReporter_12A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
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60574pIntegrase_2plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60575pIntegrase_3plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60576pIntegrase_4plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60577pIntegrase_5plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60578pIntegrase_7plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60579pIntegrase_8plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60580pIntegrase_9plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60581pIntegrase_10plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60582pIntegrase_11plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60583pIntegrase_12plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60584pIntegrase_13plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad
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60585pMemoryArrayAn array constructed by concatenating the attB/P sites of the 11 phage integrases and can be used to distinguish 2048 possible combinations of events.
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60586pCis_2+5a 2-integrase plasmid
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60587pCis_7+8a 2-integrase plasmid
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60588pCis_7+8+10a 3-integrase plasmid
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60589pCis_2+7+8+5a 4-integrase plasmid
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60590pAND_Int2A transcriptional AND circuit constructed by layering NOR and NOT gates
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60591pAND_ReporterAND gate reporter
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60592pAND_yfpused with pSpec to generate an AND gate without memory
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60593pSpecused with pAND_YFP to generate an AND gate without memory
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60594pCas_2+5encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2
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60595pCas_5+7_gfpencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.
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62848pReporter_13_switchBPA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). attP upstream of attB in this plasmid.
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Antibodies from Article