Fluorescent protein engineering by in vivo site-directed mutagenesis.
Valledor M, Hu Q, Schiller P, Myers RS
IUBMB Life. 2012 Aug;64(8):684-9. doi: 10.1002/iub.1041. Epub 2012 May 28. PubMed Article
Valledor M, Hu Q, Schiller P, Myers RS
IUBMB Life. 2012 Aug;64(8):684-9. doi: 10.1002/iub.1041. Epub 2012 May 28. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63215 | pDual-eGFP | Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells. |
| 63217 | pDual-eGFP(W66) | Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells. |
| 63218 | pDual-eGFP(Stop66) | Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells. |
| 63558 | pDual-eGFP(H66) | Expression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells. |
| 63559 | pDual-eGFP(Y203) | Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells. |