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RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling.
Mattiroli F, Vissers JH, van Dijk WJ, Ikpa P, Citterio E, Vermeulen W, Marteijn JA, Sixma TK
Cell. 2012 Sep 14;150(6):1182-95. doi: 10.1016/j.cell.2012.08.005.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
63560pCDNA3.1-Flag-H2AExpression of human H2A tagged with Flag
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63562pCDNA3.1-Flag-H2A K5-9RExpression of human H2A with K5R and K9R tagged with Flag
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63563pCDNA3.1-Flag-H2A K13-15RExpression of human H2A with K13R and K15R tagged with Flag
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63564pCDNA3.1-Flag-H2A K118-119RExpression of human H2A with K118R and K119R tagged with Flag
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63565pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129RExpression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag
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63566pCDNA3.1-Flag-H2A K13RExpression of human H2A with K13R tagged with Flag
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63567pCDNA3.1-Flag-H2A K15RExpression of human H2A with K15R tagged with Flag
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63568pCDNA3.1-Flag-H2A K13-15-118-119RExpression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag
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63570pETNKI-his-SUMO-RNF168ring (1-189)Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO
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Antibodies from Article