Plasmid Kits
Addgene distributes 162 plasmid kits — sets of related plasmids offered at a reduced cost. These include toolkits designed for modular cloning applications, expression constructs for fusion proteins, starter kits for working with certain model organisms, genome engineering platforms, and more. Find the right kit for your next experiment below.
- Gene Expression
- Genome Editing
- Cloning & SynBio
- Barcoding
- Strain Collections
- For the Classroom
- Resources
Gene Expression and Labeling
Browse kits for expressing or tagging proteins of interest, including collections of pathways and other ORFs, fluorescent reporters, and more.
| ID | Kit | Description | Vector Type | Items | Industry | PI | |
|---|---|---|---|---|---|---|---|
| 1000000088 | biGBac Kit | Rapid generation of large baculoviral transfer vectors coding for up to 25 protein complex subunits for expression in baculovirus-infected insect cells. | Insect Expression | 10 | No | Jan-Michael Peters | |
| 1000000098 | EasyClone-MarkerFree Vector Set | Plasmids for introduction of genes into 11 predetermined yeast chromosomal loci using CRISPR/Cas9. | Yeast Expression | 29 | No | Irina Borodina | |
| 1000000107 | pGCS Vector Kit | Gateway destination vectors for high-level expression of mRNAs or proteins in a variety of vertebrate organisms. | Mammalian Expression, Zebrafish Expression, Other | 12 | No | Hai-Ning Du | |
| 1000000120 | AID* Kit | Plasmid for fusion of a protein of interest to the auxin-inducible degron to control protein levels in vivo. | Yeast Expression | 18 | No | Helle Ulrich | |
| 1000000140 | EasyCloneYALI: Integrative Vector Set | Integrative plasmids for overexpression of heterologous genes in Yarrowia lipolytica. | Yeast Expression | 26 | No | Irina Borodina | |
| 1000000147 | 2in1 Plasmid Toolkit: rBiFC Kit | Plasmids for analysis of protein-protein interactions in planta via ratiometric Bimolecular Fluorescence Complementation (rBiFC). | Plant Expression | 6 | No | Christopher Grefen | |
| 1000000148 | 2in1 Plasmid Toolkit: EGFP/mCherry FLIM/FRET Kit | Plasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using EGFP and mCherry. | Plant Expression | 6 | No | Christopher Grefen | |
| 1000000149 | 2in1 Plasmid Toolkit: pmTRQ2/mVenus FLIM/FRET Kit | Plasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using pmTRQ2 and mVenus. | Plant Expression | 6 | No | Christopher Grefen | |
| 1000000150 | 2in1 Plasmid Toolkit: mVenus/tagRFP FLIM/FRET Kit | Plasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using mVenus and TagRFP. | Plant Expression | 6 | No | Christopher Grefen | |
| 1000000099 | Hsa21 Gene Expression Set | Set of cDNAs representing 162 conserved genes from the human chromosome 21 (Hsa21) for Down syndrome research. | Mammalian Expression | 163 | Yes | Roger Reeves | |
| 1000000143 | Minimal Antibiotic Resistance Platform (ARP) | Plasmids encoding resistance genes that target 17 of the most commonly found natural antibiotics. | Bacterial Expression | 15 | No | Gerard Wright | |
| 1000000163 | TRUPATH | BRET biosensor plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. | Mammalian Expression | 20 | No | Bryan Roth | |
| 1000000176 | AdenoBuilder Toolkit | Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction. | Mammalian Expression, Adenoviral | 13 | No | Fred Bunz | |
| 1000000258 | Protein Purification Optimization Kit Using SUMO Protease Elution | Validation set of recombinant proteins for high-throughput expression and purification in E. coli. | Bacterial Expression | 96 | Yes | Christopher Bahl | |
| 1000000037 | BIOFAB Plasmid Set | Collection of precisely quantitated bacterial transcription and translation initiation elements for controlled and reliable expression in E. coli. | Bacterial Expression | 49 | No | Drew Endy | |
| 1000000164 | Core Optogenetic Vector Collection (OVC) | Use the Core Optogenetic Vector Collection (OVC) to rapidly generate custom-made optogenetic tools in configurations ABC, BAC, or ACB to regulate protein-protein interactions. | Mammalian Expression | 25 | No | Harald Janovjak | |
| 1000000165 | Extended Optogenetic Vector Collection (OVC) | Expansion kit for #1000000164 includes alternative linker configurations in site B and light-activated Opto-caspase 9 constructs. | Mammalian Expression | 17 | No | Harald Janovjak | |
| 1000000211 | FNL Combinatorial Cloning Platform | A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery. | Bacterial Expression, Mammalian Expression, Insect Expression | 113 | No | Dominic Esposito | |
| 1000000133 | GoldenPiCS Kit | A flexible modular system for advanced strain engineering in Pichia pastoris to accomplish pathway expression, protein production, or other DNA integration applications. | Yeast Expression | 62 | No | Brigitte Gasser, Diethard Mattanovich, Michael Sauer | |
| 1000000144 | UbiGate Components, Set 1 | UbiGate Components (set 1) enables the reconstitution of the ubiquitination cascades of different organisms, including A. thaliana, in E. coli by 'Golden Gate' cloning. | Bacterial Expression | 86 | No | Marco Trujillo | |
| 1000000137 | Marionette Sensor Collection | This plasmid collection enables the modular control of up to 12 genes, by placing each one under the control of a small inducible promoter. | Bacterial Expression | 29 | No | Christopher Voigt | |
| 1000000157 | Yeast GPCR-sensor Toolkit | This toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors in yeast. | Yeast Expression | 42 | No | Tom Ellis | |
| 1000000242 | Ubiquiton In Vitro Kit | Plasmids for inducible, linkage-specific polyubiquitylation of a protein of interest for in vitro experiments. | In Vitro Expression | 17 | No | Helle Ulrich | |
| 1000000243 | Ubiquiton Yeast Kit | Plasmids for inducible, linkage-specific polyubiquitylation of a protein of interest in yeast. | Yeast Expression | 69 | No | Helle Ulrich | |
| 1000000244 | Ubiquiton Human Kit | Plasmids for inducible, linkage-specific polyubiquitylation of a protein of interest in human cells. | Mammalian Expression | 26 | No | Helle Ulrich | |
| 1000000011 | Yeast Gateway Kit | Destination vectors for tagging and expression of genes in S. cerevisiae. | Yeast Expression | 283 | No | Susan Lindquist | |
| 1000000058 | pCri System | Plasmids used for cytoplasmic and periplasmic/extracellular heterologous protein expression in E. coli, Bacillus subtilis, and Pichia pastoris. | Bacterial Expression, Yeast Expression | 28 | No | F. Xavier Gomis-Rüth | |
| 1000000226 | PermaPhos Kit | Plasmids to express proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) residues in E. coli. | Bacterial Expression | 5 | No | Ryan Mehl, Richard Cooley | |
| 1000000004 | Integrated Genomics: A Discovery-Based Laboratory Course | Plasmids for experiments with C. elegans and yeast in a discovery-based genomics educational course. | Worm Expression, Yeast Expression | 13 | No | Guy Caldwell | |
| 1000000022 | Open Source Wnt Project Plasmids | Plasmids containing ORFs of every human Wnt ligand, including modified versions that enable epitope tagging. | Mammalian Expression | 95 | No | Marian Waterman, Xi He, David Virshup | |
| 1000000065 | Alexandrov Cell-Free Protein Expression Kit (PEK) | Plasmids consisting of open reading frames cloned into the pCellFree vector, which enables protein expression in any in vitro translation system. | In Vitro Expression | 91 | No | Kirill Alexandrov | |
| 1000000068 | PRESTO-Tango GPCR Kit | Reporters to measure receptor activation for G protein-coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay. | Mammalian Expression | 312 | No | Bryan Roth | |
| 1000000070 | RAS Pathway 2.0 WT Clone Collection | Gateway entry clones containing expression vectors for RAS pathway proteins for in vitro and in vivo research. | Mammalian Expression | 360 | No | Dominic Esposito | |
| 1000000089 | RAS Mutant Clone Collection | Gateway entry clones containing expression vectors for RAS protein mutants for in vitro and in vivo research. | Mammalian Expression | 61 | No | Dominic Esposito | |
| 1000000072 | Cancer Pathways ORFs Kit | Vectors containing wild-type and mutant ORFs for studying and screening oncogenic signaling pathways. | Mammalian Expression, Lentiviral | 49 | No | Kris Wood, David Sabatini | |
| 1000000085 | Yeast Prototrophy Kit | Plasmids designed to restore prototrophy and test metabolic capacity in common S. cerevisiae laboratory strains. | Yeast Expression | 23 | No | Markus Ralser | |
| 1000000094 | Human Kinase Domain Constructs | A collection of His-tagged human kinase constructs that have been optimized for automated bacterial expression. | Bacterial Expression | 70 | No | Markus Seeliger, Nicholas Levinson, John Chodera | |
| 1000000103 | Broad Target Accelerator Pan-Cancer Mutant Collection | Gateway entry vectors containing wildtype and mutant alleles found across cancer types for "pan-cancer" analyses. | Mammalian Expression | 630 | No | Jesse Boehm, William Hahn, Matthew Meyerson, David Root | |
| 1000000104 | Broad Target Accelerator Lung Cancer Mutant Collection | Gateway entry vectors containing wildtype and mutant alleles associated with lung adenocarcinoma. | Mammalian Expression | 306 | No | Jesse Boehm, William Hahn, Matthew Meyerson, David Root | |
| 1000000105 | Broad Target Accelerator Genetic Pathway Reference Set | Gateway entry vectors containing wildtype and mutant proteins involved in various cellular signaling pathways and processes. | Mammalian Expression | 201 | No | Jesse Boehm, William Hahn, Matthew Meyerson, David Root | |
| 1000000223 | CEAS (C-SWAT for Essential Allele Strains) Kit | Vectors used to lower gene expression in S. cerevisiae. Compatible with the genome-wide S. cerevisiae C-SWAT strain collection. | Yeast Expression | 12 | No | Rob Mitra | |
| 1000000021 | cpFRET - FRET Based Biosensors Kit | Generate and optimize genetically encoded, unimolecular FRET biosensors for ratiometric measurements of ERK. | Mammalian Expression, Bacterial Expression, Insect Expression | 50 | No | Olivier Pertz | |
| 1000000033 | Fluorescent Protein Fusion Construct Set | Generate N- and C-terminal fusions to mCherry, Cerulean, mCitrine, or EGFP for screening fluorescent protein fusion expression in organisms or cells including Zebrafish, Xenopus, and C. elegans. | Worm Expression, Zebrafish Expression, Other | 9 | No | Amro Hamdoun | |
| 1000000168 | Plasmid-ID Kit | High-throughput assessment of competitive and effective rhizobial strains for assessing plant-bacterial interactions. | Bacterial Expression | 95 | No | Philip Poole | |
| 1000000174 | Chromoprotein Kit 1.0 | Expression of visible chromoproteins in E. coli. | Bacterial Expression | 11 | No | Anthony Forster | |
| 1000000198 | Chromoprotein Kit 2.0 | Expression of visible chromoproteins in E. coli, including two new chromoprotein genes and improved stability. | Bacterial Expression | 9 | No | Anthony Forster | |
| 1000000175 | Plasmids for Multiplexed Optical Sensors Arrayed in Islands of Cells (MOSAIC) | Plasmids and Gateway entry vectors for fluorescent sensors of diverse physiological parameters in cells. | Mammalian Expression, Lentiviral | 35 | No | Adam Cohen | |
| 1000000224 | ONE-GO Biosensors Kit | One-vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by GPCRs. | Mammalian Expression, Lentiviral | 10 | Yes | Mikel Garcia-Marcos | |
| 1000000222 | MoBiFC Kit | Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants. | Plant Expression | 50 | No | Benjamin Field | |
| 1000000215 | ScEnSor Kit | To investigate the intracellular environment of S. cerevisiae with a variety of biosensors. | Yeast Expression | 36 | No | Lisbeth Olsson | |
| 1000000039 | FX Cloning System | Use fragment exchange (FX) cloning to generate constructs for screening protein expression in several organisms with a variety of N- and C-terminal tags. | Bacterial Expression, Mammalian Expression, Yeast Expression, Insect Expression, Other | 35 | No | Raimund Dutzler, Eric Geertsma | |
| 1000000249 | T2A Split/Link TurboID Kit | Modular assembly of expression vectors for proximity labeling experiments. | Mammalian Expression, Lentiviral | 27 | No | Sven Eyckerman | |
| 1000000020 | Phosphoprotein Synthesis Kit | Engineered system for specific incorporation of phosphoserine into protein of interest. | Bacterial Expression | 7 | No | Jesse Rinehart, Dieter Söll | |
| 1000000009 | Vaccinia Virus ORF Library | A set of plasmids containing every open reading frame of the vaccinia virus. | Mammalian Expression | 253 | No | Jack Bennink, Jon Yewdell | |
| 1000000014 | Human Kinase ORF Kit | Plasmids for over 500 human kinases and kinase-related proteins in Gateway entry vectors. | Mammalian Expression | 554 | No | William Hahn, David Root | |
| 1000000012 | Myristoylated Kinase Library Kit | Plasmids for human kinases and kinase-related ORFs, with myristoylation sequence and flag-epitope tag to each. | Mammalian Expression, Retroviral | 187 | No | William Hahn, Jean Zhao | |
| 1000000001 | Fire Lab C. elegans Vector Kit | Large set of vectors for C. elegans research, including tools for RNAi, lacZ and/or fluorescent protein fusion vectors. | Worm Expression | 288 | No | Andrew Fire | |
| 1000000160 | Sybody Generation Toolbox | Plasmids to generate synthetic nanobodies against soluble proteins, membrane proteins, and protein complexes. | Bacterial Expression | 14 | No | Markus Seeger | |
| 1000000261 | Phosphoinositide Biosensors Kit | Recombinant biosensors for detection of the whole family of phosphoinositides in fixed cells and tissues. | Mammalian Expression, Insect Expression | 7 | No | David Murray | |
| 1000000267 | pLS Series: Minimal Shuttle Vectors for Saccharomyces cerevisiae | Minimal shuttle vectors of reduced size for E. coli and S. cerevisiae. | Bacterial Expression, Yeast Expression | 12 | No | Sahand Jamal Rahi | |
| 1000000271 | TCR Rapid Assembly for Functional Testing (TCRAFT) | Plasmids to efficiently assemble paired αβ T-cell receptor (TCR) libraries from sequences. | Mammalian Expression | 94 | No | Michael Birnbaum |
Genome Editing
Browse kits for editing genomes and targeting genes of interest.
| ID | Kit | Description | Editing Type |
Vector Type | Items | Industry | PI | |
|---|---|---|---|---|---|---|---|---|
| 1000000141 | EasyCloneYALI: CRISPR/Cas9 Set | Plasmids for CRISPR-based genome editing in Yarrowia lipolytica. | CRISPR | Yeast Expression | 15 | No | Irina Borodina | |
| 1000000151 | CRISPR-Cas9 Gateway System for Physcomitrella patens | System for genome editing of the moss P. patens. | CRISPR | Plant Expression | 24 | No | Magdalena Bezanilla | |
| 1000000154 | GeneWeld Kit | Targeted genome integration in human cells, zebrafish embryos, and pig fibroblasts. | CRISPR | Mammalian Expression, Zebrafish Expression | 45 | No | Jeffrey Essner | |
| 1000000159 | MoClo CRISPR/Cas Toolkit for Plants | Plasmids for gene editing using Cas nucleases and base editors in monocots and dicots. | CRISPR | Plant Expression | 95 | No | Vladimir Nekrasov | |
| 1000000171 | zCas9i Cloning Kit | MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants. | CRISPR | Plant Expression | 73 | No | Sylvestre Marillonnet, Johannes Stuttmann | |
| 1000000177 | Pro-Code Vector Kit | Lentiviral plasmids with unique protein barcodes and gRNA cloning sites for CRISPR screens and tracking cell populations. Part of the Pro-Code Vector Kits. | CRISPR | Mammalian Expression, Lentiviral | 120 | No | Brian Brown | |
| 1000000197 | Nuclear Pro-Code Vector Kit | Lentiviral plasmids with unique protein barcodes and gRNA cloning sites for CRISPR screens and tracking cell populations. Barcodes are linked to a nuclear-localizing mCherry fluorescent reporter. Part of the Pro-Code Vector Kits. | CRISPR | Mammalian Expression, Lentiviral | 75 | No | Brian Brown | |
| 1000000189 | NT-CRISPR Plasmid Collection | Plasmids for genome editing in Vibrio natriegens | CRISPR | Bacterial Expression | 11 | No | Anke Becker | |
| 1000000210 | MyLO CRISPR-Cas9 Toolkit | Markerless manipulations of the yeast genome using CRISPR/Cas9 for protein localization and overexpression | CRISPR | Yeast Expression | 96 | No | Vincent Martin | |
| 1000000219 | GFP-itis Bacterial Base Editor Activity Kit | Plasmids for a base editing experiment to correct a mutation in the EGFP fluorescent reporter gene in E. coli. | CRISPR | Bacterial Expression | 3 | No | Alexis Komor | |
| 1000000225 | CRIMPkit | Universal and highly efficient CRISPR/Cas9 targeted insertional mutagenesis system for zebrafish. | CRISPR | Zebrafish Expression | 30 | No | Robert Bryson-Richardson | |
| 1000000235 | CRISPRecise Kit | Set of high-fidelity SpCas9 variants that can be used to efficiently edit targets with practically no detectable off-target effects. | CRISPR | Mammalian Expression | 18 | No | Ervin Welker | |
| 1000000155 | pMVP and pMAGIC Cloning Systems | For rapid modular cloning of adenoviral, lentiviral, PiggyBac and Sleeping Beauty transposon vectors for transgene or RNAi delivery (pMVP) and dCas9-based epigenetic engineering (pMAGIC). | CRISPR | Mammalian Expression, Lentiviral, Adenoviral | 192 | No | Christopher Newgard | |
| 1000000191 | Fungal Toolkit for Modular Cloning (FTK) | Synthetic biology entry vectors with ready-to-use fungal genetic parts for rapid construction of genetic circuits as well as CRISPR components for efficient genome engineering in filamentous fungi. | CRISPR | Fungal Expression | 96 | No | Arnold Driessen | |
| 1000000230 | Easy-MISE Toolkit | Easy-Modular Integrative fuSion-ready Expression toolkit allows fast metabolic engineering in S. cerevisiae using CRISPR-Cas9 to insert TUs into well-defined genomic loci. | CRISPR | Yeast Expression | 49 | No | Paola Branduardi | |
| 1000000241 | GreenGate 2.0 Toolkit | Compatible with GreenGate Cloning System (#1000000036). Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and fluorescent plasma membrane and nuclear markers. | CRISPR | Plant Expression | 68 | No | Alexis Maizel | |
| 1000000257 | Expanded CRISPEY Kit | Use the Expanded CRISPEY Kit for CRISPR-based gene editing of the S. cerevisiae genome. | CRISPR | Yeast Expression | 19 | No | Jeffrey Lewis | |
| 1000000034 | Musunuru/Cowan Lab TALEN Kit | This collection allows the efficient assembly of TALEN constructs with custom repeat arrays, each containing 15 repeats for expression in mammalian cells. | TALEN | Mammalian Expression | 834 | No | Kiran Musunuru, Chad Cowan | |
| 1000000017 | REAL Assembly TALEN Kit | Restriction Enzyme And Ligation (REAL) TALEN assembly of TALE repeat arrays. Includes vector backbones for mammalian expression or in vitro transcription to produce TALE nuclease mRNAs. | TALEN | Mammalian Expression, In Vitro Transcription | 32 | No | Keith Joung | |
| 1000000024 | Golden Gate TALEN and TAL Effector Kit 2.0 | Assemble TALEN constructs with custom repeat arrays between 12 and 31 repeats via Golden Gate cloning for expression in multiple systems. | TALEN | Yeast Expression, Plant Expression, Mammalian Expression | 88 | No | Daniel Voytas, Adam Bogdanove | |
| 1000000043 | Platinum Gate TALEN Kit | Construct Platinum TALEN vectors, with variable TALE repeats and non-RVD variations for expression in cultured cells, nematodes, sea urchins, ascidians, newts, frogs, mice, or rats. | TALEN | Other | 35 | No | Takashi Yamamoto | |
| 1000000019 | TALE Toolbox | A complete set of plasmids, optimized for human expression, to build your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications. | TALEN | Mammalian Expression | 12 | No | Feng Zhang | |
| 1000000023 | LIC TAL Effector Assembly Kit | A TALE repeat unit library of 2-mer fragments that can be assembled via Ligation-independent cloning (LIC) into mammalian expression-ready TALEN constructs in 2 hierarchical assembly steps. | TALEN | Mammalian Expression | 76 | No | Veit Hornung | |
| 1000000038 | Ekker Lab TALEN Kit | Complete collection for assembly of highly active 15-RVD GoldyTALENs for expression in zebrafish. The collection complements the Golden Gate TALEN and TAL Effector Kit 2.0 (#1000000024). | TALEN | Zebrafish Expression | 256 | No | Stephen Ekker | |
| 1000000030 | Golden Gate TALEN Accessory Pack | Supplemental kit for #1000000024 includes modified pFUS vectors to simplify Golden Gate assembly and mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains. | TALEN | Mammalian Expression, In Vitro Transcription | 9 | No | Takashi Yamamoto | |
| 1000000063 | FusX TALEN assembly system | Streamlined Golden Gate TALE assembly of 15–16 RVD TALEN inserts in one step that is backwards compatible with popular TALE backbones for expression in multiple systems; requires additional plasmids for use. | TALEN | Zebrafish Expression | 336 | No | Stephen Ekker | |
| 1000000076 | GoldenBraid 2.0 Kit | Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts. | CRISPR | Plant Expression | 94 | No | Diego Orzaez | |
| 1000000055 | Multiplex CRISPR/Cas9 Assembly Systems | Plasmids to create all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs for genome engineering using Cas9 nuclease or Cas9 D10A nickase. | CRISPR | Mammalian Expression | 18 | No | Takashi Yamamoto | |
| 1000000062 | Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack | Plasmids to create all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs for genome engineering using dCas9 and FokI-dCas9. The Multiplex CRISPR/Cas9 Assembly System Kit (kit #1000000055) is required for the use of this accessory pack. | CRISPR | Mammalian Expression | 12 | No | Takashi Yamamoto | |
| 1000000077 | SapTrap CRISPR/Cas Toolkit | A modular toolkit for building CRISPR targeting vectors to insert genetic tags in the C. elegans genome. | CRISPR | Worm Expression | 26 | No | Erik Jorgensen | |
| 1000000079 | CRISPRainbow Multiplex Labeling Kit | Plasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci. | CRISPR | Mammalian Expression, Lentiviral | 14 | No | Thoru Pederson | |
| 1000000084 | pDGE Dicot Genome Editing Kit | Plasmids to assemble constructs for genome editing in dicotyledonous plants. | CRISPR | Plant Expression | 41 | No | Johannes Stuttmann | |
| 1000000086 | CRISPaint Kit | CRISPR-assisted insertion tagging (CRISPaint) plasmid collection for modular integration of large DNA fragments into user-defined genomic locations via a ligase-4-dependent mechanism. | CRISPR | Mammalian Expression | 44 | No | Veit Hornung | |
| 1000000109 | SapTrap-SEC kit for C. elegans genome engineering | A collection of C. elegans plasmids used for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC), based on the SapTrap CRISPR/Cas Toolkit. | CRISPR | Worm Expression | 14 | No | Bob Goldstein | |
| 1000000118 | pCut Plasmid Toolkit | Plasmids for integrating into well-characterized chromosomal loci to program gene expression in S. cerevisiae. | CRISPR | Yeast Expression | 26 | No | Aindrila Mukhopadhyay | |
| 1000000136 | CRISPi Kit | Plasmids for performing CRISPR/Cas9 mediated gene and genome editing in Pichia pastoris. | CRISPR | Yeast Expression | 9 | No | Brigitte Gasser | |
| 1000000051 | PUF Assembly Kit | Assembly of Pumilio and FBF (PUF) homology domain-based RNA-binding proteins for recognition of RNA targets. | PUF | Bacterial Expression, Mammalian Expression | 39 | No | Huimin Zhao | |
| 1000000237 | Fragmid | Toolkit for rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms. | CRISPR | Mammalian Expression, Lentiviral, AAV, Insect Expression | 180 | No | John Doench | |
| 1000000255 | TUNEYALI Kit | Regulate gene expression by combining gRNA constructs targeting natural promoters and insertion of synthetic promoters in Yarrowia lipolytica. | CRISPR | Yeast Expression | 64 | No | Irina Borodina | |
| 1000000015 | dRMCE Plasmid Kit | Dual recombinase-mediated cassette exchange (dRMCE) to re-engineer conditional alleles in mouse embryonic stem cells. | Recombinase | Mammalian Expression | 8 | No | Rolf Zeller | |
| 1000000031 | C. elegans miniMos Transgenesis Kit | Transgene insertion in C. elegans using a modified Mos1 transposon. | Transposon | Worm Expression | 28 | No | Erik Jorgensen | |
| 1000000117 | STAR TALE Assembly Kit | Simple TAL effector assembly reaction (STAR) for construction of TALENs, TALE-TFs and TALE-CEs. | TAL | Mammalian Expression | 84 | No | Steve Pollard | |
| 1000000212 | DdCBE Assembly Kit | Generate DddA-derived cytosine base editors (DdCBEs). | Base Editing | Zebrafish Expression | 192 | No | Bin Shen | |
| 1000000018 | ZFN Modular Assembly Kit | Engineering zinc finger arrays by modular assembly. | ZFN | Zebrafish Expression | 73 | No | Scot Wolfe, Nathan Lawson | |
| 1000000005 | Zinc Finger Consortium Modular Assembly Kit | Plasmids and strain to assemble Zinc finger nucleases (ZFNs) for performing genome engineering in a variety of organisms. | ZFN | Mammalian Expression, Bacterial Expression, Insect Expression, Plant Expression | 149 | No | Keith Joung, Daniel Voytas | |
| 1000000010 | Zinc Finger Consortium Modular Assembly Accessory Reagents Kit | Plasmids and strain for the bacterial two-hybrid system used to screen Zinc finger nucleases (ZFNs). | ZFN | Mammalian Expression, Bacterial Expression, Insect Expression, Plant Expression | 6 | No | Keith Joung, Daniel Voytas | |
| 1000000013 | Zinc Finger Consortium OPEN Reagents | Plasmids and strains used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method. | ZFN | Mammalian Expression, Bacterial Expression, Insect Expression, Plant Expression | 8 | No | Keith Joung | |
| 1000000116 | B. subtilis CRISPRi Essential Gene Knockdown Library | A collection of Bacillus subtilis strains in which each essential gene is knocked down with CRISPRi. | CRISPR | Bacterial Expression | 384 | No | Carol Gross |
Cloning and Synthetic Biology
Browse kits for assembling genetic parts to generate expression constructs, especially toolkits using standardized, modular architectures.
| ID | Kit | Description | Cloning Type |
Vector Type | Items | Industry | PI | |
|---|---|---|---|---|---|---|---|---|
| 1000000098 | EasyClone-MarkerFree Vector Set | Plasmids for introduction of genes into 11 predetermined yeast chromosomal loci using CRISPR/Cas9. | USER | Yeast Expression | 29 | No | Irina Borodina | |
| 1000000119 | Extensible Mammalian Modular Assembly (EMMA) Toolkit | Collection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids. | Golden Gate | Mammalian Expression | 95 | No | Yizhi Cai | |
| 1000000134 | Mobius Assembly Vector Toolkit | Mobius Assembly is a Golden Gate-based cloning framework which renders both high cloning capacity and vector toolkit simplicity. | Golden Gate | Bacterial Expression | 16 | No | Naomi Nakayama | |
| 1000000140 | EasyCloneYALI: Integrative Vector Set | Integrative plasmids for overexpression of heterologous genes in Yarrowia lipolytica. | USER | Yeast Expression | 26 | No | Irina Borodina | |
| 1000000141 | EasyCloneYALI: CRISPR/Cas9 Set | Plasmids for CRISPR-based genome editing in Yarrowia lipolytica. | USER | Yeast Expression | 15 | No | Irina Borodina | |
| 1000000142 | Ralstonia-GG-Kit | Plasmids for gene knockout and complementation in the bacterial plant pathogen Ralstonia solanacearum. | Golden Gate | Bacterial Expression | 17 | No | Thomas Lahaye | |
| 1000000147 | 2in1 Plasmid Toolkit: rBiFC Kit | Plasmids for analysis of protein-protein interactions in planta via ratiometric Bimolecular Fluorescence Complementation (rBiFC). | Gateway | Plant Expression | 6 | No | Christopher Grefen | |
| 1000000148 | 2in1 Plasmid Toolkit: EGFP/mCherry FLIM/FRET Kit | Plasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using EGFP and mCherry. | Gateway | Plant Expression | 6 | No | Christopher Grefen | |
| 1000000149 | 2in1 Plasmid Toolkit: pmTRQ2/mVenus FLIM/FRET Kit | Plasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using pmTRQ2 and mVenus. | Gateway | Plant Expression | 6 | No | Christopher Grefen | |
| 1000000150 | 2in1 Plasmid Toolkit: mVenus/tagRFP FLIM/FRET Kit | Plasmids for analysis of protein-protein interactions in planta via Förster Resonance Energy Transfer (FRET) using mVenus and tagRFP. | Gateway | Plant Expression | 6 | No | Christopher Grefen | |
| 1000000159 | MoClo CRISPR/Cas Toolkit for Plants | Plasmids for gene editing using Cas nucleases and base editors in monocots and dicots. | Golden Gate (MoClo) | Plant Expression | 95 | No | Vladimir Nekrasov | |
| 1000000171 | zCas9i Cloning Kit | MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants. | Golden Gate (MoClo) | Plant Expression | 73 | No | Sylvestre Marillonnet, Johannes Stuttmann | |
| 1000000176 | AdenoBuilder Toolkit | Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction. | Gibson | Mammalian Expression, Adenoviral | 13 | No | Fred Bunz | |
| 1000000044 | MoClo Toolkit | Collection of standardized genetic modules that can be used for hierarchical assembly of multi-gene expression constructs. | Golden Gate (MoClo) | Eukaryotic Expression | 95 | Yes | Sylvestre Marillonnet | |
| 1000000061 | MoClo-YTK | Collection of standardized parts for hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. | Golden Gate (MoClo) | Yeast Expression | 96 | No | John Dueber | |
| 1000000059 | CIDAR MoClo Parts Kit | Collection of standardized parts for combinatorial design and expression tuning in E. coli. | Golden Gate (MoClo) | Bacterial Expression | 91 | No | Douglas Densmore | |
| 1000000217 | AspFlex Kit | Collection of standardized parts for protein expression analyses and CRISPRi experiments in Acinetobacter sp. | Golden Gate (MoClo) | Bacterial Expression | 19 | No | Veronica Godoy-Carter | |
| 1000000233 | Joint Modular Cloning (JMC) Toolkit | Modular cloning chassis and set of functional parts for assembling plant transformation vectors, compatible with MoClo. | Golden Gate | Plant Expression | 76 | No | Daniel Voytas | |
| 1000000229 | Multiplex Yeast Toolkit (MYT) | Collection of plasmids which extend the capabilities of the MoClo Yeast Toolkit (YTK) for genetically engineering S. cerevisiae. | Golden Gate (MoClo) | Yeast Expression | 96 | No | Tom Ellis | |
| 1000000236 | MoClo Plant Parts III: Transformation & Genome Engineering Kit | Collection of MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly. | Golden Gate (MoClo) | Plant Expression | 94 | No | Daniel Voytas | |
| 1000000153 | MetClo DNA Assembly Kit | Methylase-assisted one-pot assembly of large DNA fragments. Compatible with other type IIS restriction enzyme-based systems. Includes three E. coli strains necessary for vector preparation. | Type IIS Restriction Enzyme | Bacterial Expression | 51 | No | Christopher A. O'Callaghan | |
| 1000000155 | pMVP and pMAGIC Cloning Systems | For rapid modular cloning of adenoviral, lentiviral, PiggyBac and Sleeping Beauty transposon vectors for transgene or RNAi delivery (pMVP) and dCas9-based epigenetic engineering (pMAGIC). | MultiSite Gateway | Mammalian Expression, Lentiviral, Adenoviral | 192 | No | Christopher Newgard | |
| 1000000164 | Core Optogenetic Vector Collection (OVC) | Use the Core Optogenetic Vector Collection (OVC) to rapidly generate custom-made optogenetic tools in configurations ABC, BAC, or ACB to regulate protein-protein interactions. | Restriction Enzyme | Mammalian Expression | 25 | No | Harald Janovjak | |
| 1000000165 | Extended Optogenetic Vector Collection (OVC) | Expansion kit for #1000000164 includes alternative linker configurations in site B and light-activated Opto-caspase 9 constructs. | Restriction Enzyme | Mammalian Expression | 17 | No | Harald Janovjak | |
| 1000000167 | Yarrowia lipolytica Golden Gate Toolkit | Standardized parts that can be used for one-step integrative vector assembly of up to three transcription units for engineering Yarrowia lipolytica strains. | Golden Gate | Yeast Expression | 64 | No | Tristan Rossignol | |
| 1000000190 | The Expanded EasyClone-MarkerFree toolkit | Expansion of the EasyClone-MarkerFree Vector Set (#1000000098) with eight additional chromosomal loci for the stable integration of heterologous DNA into S. cerevisiae by CRISPR/Cas9. | USER | Yeast Expression | 16 | No | Irina Borodina | |
| 1000000191 | Fungal Toolkit for Modular Cloning (FTK) | Synthetic biology entry vectors with ready-to-use fungal genetic parts for rapid construction of genetic circuits as well as CRISPR components for efficient genome engineering in filamentous fungi. | Golden Gate | Fungal Expression | 96 | No | Arnold Driessen | |
| 1000000211 | FNL Combinatorial Cloning Platform | A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery. | MultiSite Gateway | Bacterial Expression, Mammalian Expression, Insect Expression | 113 | No | Dominic Esposito | |
| 1000000221 | High Complexity Golden Gate Assembly Standards Kit | End-joining ligation fidelity and bias data predicts high accuracy junction sets for Golden Gate assembly, providing a positive control for complex assembly via blue/white screening in E. coli. | Golden Gate | Bacterial Expression | 25 | Yes | Eric Cantor | |
| 1000000230 | Easy-MISE Toolkit | Easy-Modular Integrative fuSion-ready Expression toolkit allows fast metabolic engineering in S. cerevisiae using CRISPR-Cas9 to insert TUs into well-defined genomic loci. | Golden Gate | Yeast Expression | 49 | No | Paola Branduardi | |
| 1000000238 | Diatom uLoop Assembly Kit | Universal Loop (uLoop) assembly system for efficient and cross-kingdom DNA assembly and to build conjugatable episomes using diatom promoters and terminators. | Golden Gate (Loop) | Yeast Expression, Plant Expression, Bacterial Expression, Other | 95 | No | Christopher Dupont | |
| 1000000241 | GreenGate 2.0 Toolkit | Compatible with GreenGate Cloning System (#1000000036). Includes modules for CRISPR/Cas9 genome editing, dexamethasone-inducible expression, and fluorescent plasma membrane and nuclear markers. | Golden Gate (GreenGate) | Plant Expression | 68 | No | Alexis Maizel | |
| 1000000024 | Golden Gate TALEN and TAL Effector Kit 2.0 | Assemble TALEN constructs with custom repeat arrays between 12 and 31 repeats via Golden Gate cloning for expression in multiple systems. | Golden Gate | Yeast Expression, Plant Expression, Mammalian Expression | 88 | No | Daniel Voytas, Adam Bogdanove | |
| 1000000043 | Platinum Gate TALEN Kit | Construct Platinum TALEN vectors, with variable TALE repeats and non-RVD variations for expression in cultured cells, nematodes, sea urchins, ascidians, newts, frogs, mice, or rats. | Golden Gate | Other | 35 | No | Takashi Yamamoto | |
| 1000000038 | Ekker Lab TALEN Kit | Complete collection for assembly of highly active 15-RVD GoldyTALENs for expression in zebrafish. The collection complements the Golden Gate TALEN and TAL Effector Kit 2.0 (#1000000024). | Golden Gate | Zebrafish Expression | 256 | No | Stephen Ekker | |
| 1000000030 | Golden Gate TALEN Accessory Pack | Supplemental kit for #1000000024 includes modified pFUS vectors to simplify Golden Gate assembly and mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains. | Golden Gate | Mammalian Expression, In Vitro Transcription | 9 | No | Takashi Yamamoto | |
| 1000000063 | FusX TALEN assembly system | Streamlined Golden Gate TALE assembly of 15–16 RVD TALEN inserts in one step that is backwards compatible with popular TALE backbones for expression in multiple systems; requires additional plasmids for use. | Golden Gate | Zebrafish Expression | 336 | No | Stephen Ekker | |
| 1000000076 | GoldenBraid 2.0 Kit | Plant synthetic biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts. | Golden Gate (GoldenBraid) | Plant Expression | 94 | No | Diego Orzaez | |
| 1000000080 | EcoFlex MoClo Kit | Collection of DNA parts for synthetic biology use in E. coli, cell-free protein synthesis, protein purification, and hierarchical assembly of transcription units. | Golden Gate | Bacterial Expression | 78 | No | Paul Freemont | |
| 1000000108 | MoClo Pichia Toolkit | A set of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit (#1000000061) for protein expression and secretion in Pichia pastoris. | MoClo | Yeast Expression | 21 | No | Volker Sieber | |
| 1000000133 | GoldenPiCS Kit | A flexible modular system for advanced strain engineering in Pichia pastoris to accomplish pathway expression, protein production, or other DNA integration applications. | Golden Gate | Yeast Expression | 62 | No | Brigitte Gasser, Diethard Mattanovich, Michael Sauer | |
| 1000000135 | MoClo Plant Parts II and Infrastructure Kit | Modules connect MoClo and Gateway for hierarchical assembly of plant multigene constructs; contains yeast two-hybrid and bacterium-to-plant translocation vectors. Partially compatible with GoldenBraid. | Golden Gate (MoClo) | Plant Expression | 96 | No | Johannes Stuttmann | |
| 1000000138 | Yeast GoldenBraid Cloning System and Toolkit | This toolkit allows generation of transcriptional units using GoldenBraid for integration in the S. cerevisiae genome at loci, YPRCΔ15 and YORWΔ22, to support high and stable transgene expression. | Golden Gate (GoldenBraid) | Yeast Expression | 31 | No | Elena Caro Bernat | |
| 1000000144 | UbiGate Components, Set 1 | UbiGate Components (set 1) enables the reconstitution of the ubiquitination cascades of different organisms, including A. thaliana, in E. coli by 'Golden Gate' cloning. | Golden Gate | Bacterial Expression | 86 | No | Marco Trujillo | |
| 1000000145 | UbiGate E2 Activity Screen, Set 2 | UbiGate E2 Activity Screen (set 2) includes expression cassettes containing ubiquitin, the E1 UBA1 in combination with all Arabidopsis thaliana E2's for E3 autoubiquitination assays. See Kit #1000000144 for Set 1 components. | Golden Gate | Bacterial Expression | 49 | No | Marco Trujillo | |
| 1000000152 | Start-Stop Assembly Toolkit | Modular, scarless DNA assembly method suitable for combinatorial assembly and ideal for metabolic engineering. | Golden Gate | Bacterial Expression | 40 | No | John Heap | |
| 1000000156 | MoChlo: Modular Cloning Chloroplast Toolbox | Library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors. | Golden Gate (MoClo) | Plant Expression | 122 | No | Scott Lenaghan | |
| 1000000157 | Yeast GPCR-sensor Toolkit | This toolkit includes plasmids for constructing highly-tunable GPCR-based biosensors in yeast. | Golden Gate (MoClo) | Yeast Expression | 42 | No | Tom Ellis | |
| 1000000234 | Cultivarium POSSUM Toolkit | Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model bacteria. | Golden Gate (Loop) | Bacterial Expression | 189 | No | Nili Ostrov, Charlie Gilbert, Cultivarium Tools | |
| 1000000240 | MoClo Yeast Secretion and Display (YSD) Toolkit | Collection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast. | Golden Gate (MoClo) | Yeast Expression | 34 | No | Paul Young | |
| 1000000251 | POMBOX MoClo YTK | Seamless and efficient assembly of single gene and multigene plasmids for genome integration in Schizosaccharomyces pombe. | Golden Gate (MoClo) | Yeast Expression | 38 | No | Tomáš Pluskal | |
| 1000000036 | GreenGate Cloning System | Plasmids for rapidly assembling plant transformation constructs, based on the Golden Gate method. | Golden Gate (GreenGate) | Plant Expression | 56 | No | Jan Lohmann | |
| 1000000054 | Golden GATEway Cloning Kit | A collection that combines Golden Gate with Multisite Gateway cloning for assembly of complex fusion proteins or transgenesis constructs. | Golden Gate, MultiSite Gateway | Zebrafish Expression, Other | 44 | No | Joachim Wittbrodt | |
| 1000000050 | MultiSite Gateway Kit | Plasmids for constructing fully-customizable multi-part fusion proteins. | MultiSite Gateway | Mammalian Expression, Other | 12 | No | Anna Planas, Tomas Santalucia | |
| 1000000011 | Yeast Gateway Kit | Destination vectors for tagging and expression of genes in S. cerevisiae. | Gateway | Yeast Expression | 283 | No | Susan Lindquist | |
| 1000000060 | Multiple Lentiviral Expression (MuLE) System Kit | A plasmid toolbox used to construct vectors for constitutive or inducible gene expression, knockdown, deletion or editing. | MultiSite Gateway | Mammalian Expression, Lentiviral | 96 | No | Ian Frew | |
| 1000000064 | MXS Chaining Kit | A collection of standardized building blocks used to assemble plasmids that are optimized for fluorescence imaging or flow cytometry applications. | Restriction Enzyme | Mammalian Expression | 55 | No | Pierre Neveu | |
| 1000000073 | EasyClone 2.0 Yeast Toolkit | An expanded toolkit of integrative vectors for stable gene expression in S. cerevisiae. | USER | Yeast Expression | 25 | No | Irina Borodina | |
| 1000000087 | Tol2 Zebrafish Neurodegenerative Disorder Toolkit | A plasmid collection, based on the Tol2kit, used to streamline the production of transgenic zebrafish for studying neurodegenerative disorders. | MultiSite Gateway | Zebrafish Expression | 19 | No | Nicholas Cole | |
| 1000000180 | The Mammalian Toolkit | Plasmids for Golden Gate-based assembly of cellular circuits for use in mammalian cell. | Golden Gate | Mammalian Expression | 253 | No | Hana El-Samad | |
| 1000000216 | Komagataeibacter Tool Kit (KTK) | A collection of standardized parts used for hierarchical assembly of single and multi-gene constructs for Komagataeibacter species. | Golden Gate (MoClo) | Bacterial Expression | 50 | No | Tom Ellis | |
| 1000000227 | openCIDAR MoClo Toolkit | A collection of standardized parts compatible with CIDAR MoClo (Addgene #1000000059) to assemble gene expression constructs in many bacteria across Pseudomonadota. | Golden Gate (MoClo) | Bacterial Expression | 28 | No | Eric Young | |
| 1000000222 | MoBiFC Kit | Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants. | Golden Gate (MoClo) | Plant Expression | 50 | No | Benjamin Field | |
| 1000000051 | PUF Assembly Kit | Assembly of Pumilio and FBF (PUF) homology domain-based RNA-binding proteins for recognition of RNA targets. | Golden Gate | Bacterial Expression, Mammalian Expression | 39 | No | Huimin Zhao | |
| 1000000039 | FX Cloning System | Use fragment exchange (FX) cloning to generate constructs for screening protein expression in several organisms with a variety of N- and C-terminal tags. | FX Cloning | Bacterial Expression, Mammalian Expression, Yeast Expression, Insect Expression, Other | 35 | No | Raimund Dutzler, Eric Geertsma | |
| 1000000245 | EXPRESSYALI | Assembly of expression constructs for Yarrowia lipolytica. | Golden Gate | Yeast Expression | 29 | No | Irina Borodina | |
| 1000000237 | Fragmid | Toolkit for rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms. | Golden Gate (MoClo) | Mammalian Expression, Lentiviral, AAV, Insect Expression | 180 | No | John Doench | |
| 1000000249 | T2A Split/Link TurboID Kit | Modular assembly of expression vectors for proximity labeling experiments. | Golden Gate | Mammalian Expression, Lentiviral | 27 | No | Sven Eyckerman | |
| 1000000250 | MultiGreen Kit | Modular assembly of multi-gene expression vectors. | Golden Gate (GreenGate) | Plant Expression | 48 | No | Wayne Parrott | |
| 1000000252 | 4G-Cloning Plasmid Kit | Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts. | Golden Gate | Bacterial Expression | 29 | No | Stephan Gruber | |
| 1000000255 | TUNEYALI Kit | Regulate gene expression by combining gRNA constructs targeting natural promoters and insertion of synthetic promoters in Yarrowia lipolytica. | Golden Gate | Yeast Expression | 64 | No | Irina Borodina | |
| 1000000035 | pOSIP Plasmid Kit | Cloning and assembly of DNA constructs for chromosomal integration in E. coli. | Clonetegration | Bacterial Expression | 11 | No | Drew Endy, Keith Shearwin | |
| 1000000146 | CyanoGate Kit | Vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria. | Golden Gate (MoClo) | Bacterial Expression | 96 | No | Alistair McCormick | |
| 1000000161 | CIDAR MoClo Extension, Volume I | Plasmids to efficiently build a wide variety of gene expression constructs. Can be used as a stand-alone toolkit or with the CIDAR MoClo Parts Kit (Addgene #1000000059). | Golden Gate (MoClo) | Bacterial Expression | 110 | No | Richard Murray | |
| 1000000166 | Yeast Secrete and Detect Kit | Assembly of transcription units for protein secretion in the yeasts S. cerevisiae and Pichia pastoris. | Golden Gate (MoClo) | Yeast Expression | 42 | No | Sylvestre Marillonnet | |
| 1000000253 | EcoFlex MoClo Extension, Volume I | Plasmids to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system (Addgene #1000000080). | Golden Gate (MoClo) | Bacterial Expression | 96 | No | Mikhail Shapiro | |
| 1000000256 | MoClo Baculo Toolkit | Plasmids to construct multigene expression vectors for yeast and baculovirus, through compatibility with the Bac-to-Bac and biGBac systems. | Golden Gate (MoClo) | Yeast Expression, Insect Expression | 41 | No | Chen Davidovich | |
| 1000000259 | XanthoMoClo Kit | Plasmids and parts for modular assembly of single and multi-gene plasmids for any Xanthobacter or Roseixanthobacter strain. | Golden Gate (MoClo) | Bacterial Expression | 161 | No | Michael Springer | |
| 1000000262 | LbPlant Toolbox I | Plasmids and parts for hierarchical assembly for expression in Lactiplantibacillus plantarum. Recommended to be used in combination with LbPlant Toolbox II. | Golden Gate (MoClo) | Bacterial Expression | 29 | Yes | Thorsten Mascher | |
| 1000000263 | LbPlant Toolbox II | Plasmids and parts for hierarchical assembly for expression in Lactiplantibacillus plantarum. Recommended to be used in combination with LbPlant Toolbox I. | Golden Gate (MoClo) | Bacterial Expression | 10 | No | Georg Fritz | |
| 1000000266 | yeast Tunable Expression Systems Toolkit (yTEST) | Plasmids for assembly of small-molecule inducible promoter systems in S. cerevisiae. | Golden Gate (MoClo) | Yeast Expression | 62 | No | Jeff Hasty | |
| 1000000268 | MoClo Yeast Secretion Biosensor (YSB) Toolkit | Library of plasmids containing a variety of signal peptides, C-tags, and alpha-mating factor tags for use in protein secretion optimization in yeast. | Golden Gate (MoClo) | Yeast Expression | 57 | No | Joseph Brock | |
| 1000000269 | CHLOROMODAS Kit | Plasmids for for engineering the Chlamydomonas reinhardtii chloroplast. | Golden Gate (MoClo) | Other | 191 | No | Tobias Erb |
Barcoding
Browse kits for identification and tracing by unique plasmid barcode sequences.
| ID | Kit | Description | Vector Type | Items | Industry | PI | |
|---|---|---|---|---|---|---|---|
| 1000000177 | Pro-Code Vector Kit | Lentiviral plasmids with unique protein barcodes and gRNA cloning sites for CRISPR screens and tracking cell populations. Part of the Pro-Code Vector Kits. | Mammalian Expression, Lentiviral | 120 | No | Brian Brown | |
| 1000000197 | Nuclear Pro-Code Vector Kit | Lentiviral plasmids with unique protein barcodes and gRNA cloning sites for CRISPR screens and tracking cell populations. Barcodes are linked to a nuclear-localizing mCherry fluorescent reporter. Part of the Pro-Code Vector Kits. | Mammalian Expression, Lentiviral | 75 | No | Brian Brown | |
| 1000000097 | Retroviral Barcoding Library | Plasmids with 6-nucleotide barcodes for in vivo phenotypic screening of small-molecule libraries. | Mammalian Expression, Retroviral | 96 | No | Monte Winslow | |
| 1000000032 | FREQ-Seq Kit: High Resolution Allele Frequency Measurements | A collection of plasmids can be used to construct barcoded, locus-specific libraries compatible with Illumina next generation sequencing. | Bacterial Expression | 48 | No | Christopher Marx | |
| 1000000213 | Barcoded Self-Reporting Transposon Calling Card Collection | A set of plasmids containing barcoded self-reporting transposons used for 'calling card' experiments to identify transcription factor binding sites and changes in gene expression. | Mammalian Expression | 43 | No | Rob Mitra |
Strain Collections
Browse kits consisting of bacterial strains.
| ID | Kit | Description | Vector Type | Items | Industry | PI | |
|---|---|---|---|---|---|---|---|
| 1000000115 | B. subtilis Single Gene Deletion Library | A collection of Bacillus subtilis strains in which each non-essential gene has been individually replaced with a kanamycin resistance cassette. | Bacterial Expression | 384 | No | Carol Gross | |
| 1000000116 | B. subtilis CRISPRi Essential Gene Knockdown Library | A collection of Bacillus subtilis strains in which each essential gene is knocked down with CRISPRi. | Bacterial Expression | 384 | No | Carol Gross |
For the Classroom
Browse kits for teaching and educational purposes.
| ID | Kit | Description | Vector Type | Items | Industry | PI | |
|---|---|---|---|---|---|---|---|
| 1000000174 | Chromoprotein Kit 1.0 | Expression of visible chromoproteins in E. coli. | Bacterial Expression | 11 | No | Anthony Forster | |
| 1000000198 | Chromoprotein Kit 2.0 | Expression of visible chromoproteins in E. coli, including two new chromoprotein genes and improved stability. | Bacterial Expression | 9 | No | Anthony Forster | |
| 1000000219 | GFP-itis Bacterial Base Editor Activity Kit | Plasmids for a base editing experiment to correct a mutation in the EGFP fluorescent reporter gene in E. coli. | Bacterial Expression | 3 | No | Alexis Komor | |
| 1000000004 | Integrated Genomics: A Discovery-Based Laboratory Course | Plasmids for experiments with C. elegans and yeast in a discovery-based genomics educational course. | Worm Expression, Yeast Expression | 13 | No | Guy Caldwell |
Other Resources
More Kit Resources
Other Addgene Resources
Content last reviewed: 24 October 2025