STICR Pooled Library
(Pooled Library #180483, #186334, #186335)

• Purpose:

  The "single-cell-RNA-sequencing-compatible tracer for identifying clonal relationships" (STICR) barcoding libraries are lentiviral vectors encoding a CAG-GFP transgene with a pre-defined, error-correctable molecular barcode within the 3' UTR. This barcode can be captured by oligo(dT)-based scRNA-seq platforms, including the 10X genomics scRNA-seq 3' platform. See schematic of barcode structure below.

  Each STICR library comprises 50-70 million unique sequences, allowing labeling of a starting cell population and clonal lineage/fate tracing. In addition, each individual library contains a unique constant "index" region within the barcode. This can be used to distinguish cells infected with different libraries.

  Index Sequences

  • Index1: ATC
  • Index3: GAG
  • IndexE: No additional sequence. It’s “index” is the absence of additional sequence.

  Note: Index2 library was designed, but not created. Index numbering was kept the same for consistency in our record keeping.

• Vector Backbone:

  pSico

• Depositing Labs: Tomasz Nowakowski
• Publication: Delgado et al. Nature. 2022 Jan; 601(7893):397-403. doi: 10.1038/s41586-021-04230-7.

Library Details

• Genes/gRNAs/plasmids: ~50-70 million plasmids / library
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ~50 uL
• Concentration: 50 ng/µL

Resource Information

• Protocols:
  • Protocol for NGS Validation of Library (16 KB)
  • Library Amplification Protocol (17 KB)
• Depositor Data:
  • STICR_FullyBarcoded_IndexE.dna
  • STICR_FullyBarcoded_Index1.dna
  • STICR_FullyBarcoded_Index3.dna
• Terms and Licenses:
  • UBMTA

Depositor Comments

User instructions are available on GitHub ( https://github.com/NOW-Lab/STICR).