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STICR Pooled Library
(Pooled Library #180483, #186334, #186335)

  • Purpose

    The "single-cell-RNA-sequencing-compatible tracer for identifying clonal relationships" (STICR) barcoding libraries are lentiviral vectors encoding a CAG-GFP transgene with a pre-defined, error-correctable molecular barcode within the 3' UTR. This barcode can be captured by oligo(dT)-based scRNA-seq platforms, including the 10X genomics scRNA-seq 3' platform. See schematic of barcode structure below.

    Each STICR library comprises 50-70 million unique sequences, allowing labeling of a starting cell population and clonal lineage/fate tracing. In addition, each individual library contains a unique constant "index" region within the barcode. This can be used to distinguish cells infected with different libraries.

    Index Sequences

    • Index1: ATC
    • Index3: GAG
    • IndexE: No additional sequence. It’s “index” is the absence of additional sequence.

    Note: Index2 library was designed, but not created. Index numbering was kept the same for consistency in our record keeping.

  • Vector Backbone


Item Catalog # Description Quantity Price (USD)
Pooled Library 180483 STICR_IndexE pooled library 1 $ 325 Add to Cart
Pooled Library 186334 STICR_Index1 pooled library 1 $ 325 Add to Cart
Pooled Library 186335 STICR_Index3 pooled library 1 $ 325 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Genes/gRNAs/plasmids
    ~50-70 million plasmids / library
  • Lentiviral Generation

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ~50 uL
  • Concentration
    50 ng/µL

Depositor Comments

STICR lentiviral vector map showing CAG, EGFP, WPRE, Primer, Viral Index, SiteBarcode Fragments, and pA

User instructions are available on GitHub (

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    STICR Pooled Library was a gift from Tomasz Nowakowski (Addgene # )
  • For your References section:

    Individual human cortical progenitors can produce excitatory and inhibitory neurons. Delgado RN, Allen DE, Keefe MG, Mancia Leon WR, Ziffra RS, Crouch EE, Alvarez-Buylla A, Nowakowski TJ. Nature. 2022 Jan; 601(7893):397-403. doi: 10.1038/s41586-021-04230-7. PubMed 26232224