STICR Pooled Libraries
(Pooled Library #180483, #186334, #186335)
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Purpose
The "single-cell-RNA-sequencing-compatible tracer for identifying clonal relationships" (STICR) barcoding libraries are lentiviral vectors encoding a CAG-GFP transgene with a pre-defined, error-correctable molecular barcode within the 3' UTR. This barcode can be captured by oligo(dT)-based scRNA-seq platforms, including the 10X genomics scRNA-seq 3' platform. See schematic of barcode structure below.
Each STICR library comprises 50–70 million unique sequences, allowing labeling of a starting cell population and clonal lineage/fate tracing. In addition, each individual library contains a unique constant "index" region within the barcode. This can be used to distinguish cells infected with different libraries.
Index Sequences
- Index1: ATC
- Index3: GAG
- IndexE: No additional sequence. It’s “index” is the absence of additional sequence.
Note: Index2 library was designed, but not created. Index numbering was kept the same for consistency in our record keeping.
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Vector Backbone
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Pooled Library | 180483 | STICR_IndexE | 1 | $325 | Add to Cart | |
Pooled Library | 186334 | STICR_Index1 | 1 | $325 | Add to Cart | |
Pooled Library | 186335 | STICR_Index3 | 1 | $325 | Add to Cart |
Library Details
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Genes/gRNAs/plasmids~50–70 million plasmids / library
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume~50 uL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
Terms and Licenses
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Academic/Nonprofit TermsIndustry Terms
- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
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These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
STICR Pooled Library was a gift from Tomasz Nowakowski (Addgene # ) -
For your References section:
Individual human cortical progenitors can produce excitatory and inhibitory neurons. Delgado RN, Allen DE, Keefe MG, Mancia Leon WR, Ziffra RS, Crouch EE, Alvarez-Buylla A, Nowakowski TJ. Nature. 2022 Jan; 601(7893):397-403. doi: 10.1038/s41586-021-04230-7. PubMed 26232224