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931 Results for:  Lambda RED

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Principal Investigators

Addgene Guides & Resources

Plasmids, Strains, and Pooled Libraries 1–20 of 928

  1. Plasmid
    #127032
    Depositor
    Purpose
    Basic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).
    Insert
    Part ?RED_NOC Synthetic
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  2. Plasmid
    #13263
    Depositor
    Insert
    Plac-gam-bet-exo bacteriophage lambda
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  3. Plasmid
    #13078
    Depositor
    Insert
    Ptac-gam-bet-exo operon bacteriophage lambda
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  4. Bacterial strain
    #68246
    Depositor
    Purpose
    E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
    Insert
    arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
    Use
    Tags
    None
    Expression
    Mutation
    None
  5. Plasmid
    #13079
    Depositor
    Insert
    Ptac-gam-bet-exo operon bacteriophage lambda
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  6. Plasmid
    #106396
    Depositor
    Purpose
    Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ
    Insert
    cas9 Streptococcus pyogenes
    Insert
    Lambda red genes Lambda bacteriophage
    Use
    CRISPR, Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  7. Plasmid
    #79967
    Depositor
    Purpose
    TM-MAGE strain
    Insert
    Lambda Red recombinase beta subunit Escherichia coli (EcNR1)
    Insert
    DNA adenine methylase Escherichia coli K-12 MG1655
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  8. Plasmid
    #62225
    Depositor
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Insert
    cas9 S. pyogenes MGAS5005
    Use
    CRISPR ; Lambda Red recombinase
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  9. Bacterial strain
    #11675
    Depositor
    Insert
    Red-alpha, Red-beta, Gam Bacteriophage lambda
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  10. Bacterial strain
    #48936
    Depositor
    Purpose
    Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Insert
    galK::fadBp-CAT, mutS::bla
    Use
    Tags
    None
    Expression
    Mutation
    None
  11. Plasmid
    #71541
    Depositor
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Insert
    Cas9 S. pyogenes
    Insert
    lambda red genes
    Insert
    plasmid curing system
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  12. Plasmid
    #79968
    Depositor
    Purpose
    TM-MAGE strain
    Insert
    Lambda Red recombinase beta subunit Escherichia coli (EcNR1)
    Insert
    DNA adenine methylase Escherichia coli K-12 MG1655
    Insert
    Levansucrase Escherichia coli (DA16543)
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  13. Plasmid
    #73950
    Depositor
    Purpose
    pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX
    Insert
    cas9 Bacteria
    Insert
    lambda beta
    Insert
    dam
    Insert
    recX
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  14. Plasmid
    #102284
    Depositor
    Purpose
    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.
    Insert
    pfkB gRNA E. coli
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  15. Plasmid
    #89959
    Depositor
    Purpose
    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.
    Insert
    lpoB gRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  16. Plasmid
    #89954
    Depositor
    Purpose
    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.
    Insert
    hisG gRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  17. Plasmid
    #102291
    Depositor
    Purpose
    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.
    Insert
    ompT gRNA
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  18. Plasmid
    #62656
    Depositor
    Purpose
    Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid
    Insert
    exo, beta, gam, sgRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
  19. Plasmid
    #72679
    Depositor
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.
    Insert
    mutL E32K Escherichia coli
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring domi...
  20. Plasmid
    #89960
    Depositor
    Purpose
    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.
    Insert
    empty gRNA
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    None
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