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Search Results 1–20 of 677

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Plasmids

  1. pTP223 (Plasmid #13263)
    Insert: Plac-gam-bet-exo bacteriophage lambda
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Kenan Murphy
  2. pKM201 (Plasmid #13078)
    Insert: Ptac-gam-bet-exo operon bacteriophage lambda
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Kenan Murphy
  3. Strain SIJ_488 (Bacterial strain #68246)
    Purpose E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
    Insert: arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Alex Nielsen
  4. pKM200 (Plasmid #13079)
    Insert: Ptac-gam-bet-exo operon bacteriophage lambda
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Kenan Murphy
  5. pMA7 (Plasmid #79967)
    Purpose TM-MAGE strain
    Insert: Lambda Red recombinase beta subunit Escherichia coli (EcNR1)
    Insert: DNA adenine methylase Escherichia coli K-12 MG1655
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Morten Sommer
  6. pCas (Plasmid #62225)
    Purpose Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Insert: cas9 S. pyogenes MGAS5005
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use: CRISPR ; Lambda Red recombinase
    Deposited by: Sheng Yang
  7. BUN21/pML300 (recipient strain) (Bacterial strain #11675)
    Insert: Red-alpha, Red-beta, Gam Bacteriophage lambda
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Stephen Elledge
  8. EcNGRfC (Bacterial strain #48936)
    Purpose Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Insert: galK::fadBp-CAT, mutS::bla
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: George Church
  9. pREDCas9 (Plasmid #71541)
    Purpose Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Insert: Cas9 S. pyogenes
    Insert: lambda red genes
    Insert: plasmid curing system
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Tao Chen
  10. pMA7-SacB (Plasmid #79968)
    Purpose TM-MAGE strain
    Insert: Lambda Red recombinase beta subunit Escherichia coli (EcNR1)
    Insert: DNA adenine methylase Escherichia coli K-12 MG1655
    Insert: Levansucrase Escherichia coli (DA16543)
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Morten Sommer
  11. pMA7CR_2.0 (Plasmid #73950)
    Purpose pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX
    Insert: cas9 Bacteria
    Insert: lambda beta
    Insert: dam
    Insert: recX
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Alex Nielsen
  12. pKDsgRNA-lpoB (Plasmid #89959)
    Purpose Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.
    Insert: lpoB gRNA
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Michael Lynch
  13. pKDsgRNA-hisG (Plasmid #89954)
    Purpose Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.
    Insert: hisG gRNA
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Michael Lynch
  14. pORTMAGE-3 (Plasmid #72678)
    Purpose Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.
    Insert: mutL E32K Escherichia coli
    Tags: none
    Expression: Bacterial
    Mutation: E32K mutation conferring domi...
    Use:
    Deposited by: Csaba Pál
  15. pKDsgRNA-xylA (Plasmid #89964)
    Purpose Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.
    Insert: xylA gRNA
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Michael Lynch
  16. pKDsgRNA-rssA (Plasmid #89957)
    Purpose Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.
    Insert: rssA gRNA
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Michael Lynch
  17. pKDsgRNA-arsB (Plasmid #89952)
    Purpose Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.
    Insert: arsB gRNA
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Michael Lynch
  18. EcNGRfEfC-fadD-tesA'-fadR (Bacterial strain #48939)
    Purpose Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Insert: galK::fadBp-CAT, mutS::bla, fadE265G>T/268delG
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: George Church
  19. pKDsgRNA-ack (Plasmid #62654)
    Purpose Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression
    Insert: exo, beta, gam, sgRNA
    Tags: None
    Expression: Bacterial
    Mutation: None
    Use:
    Deposited by: Kristala Prather
  20. pORTMAGE-2 (Plasmid #72677)
    Purpose Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Insert: mutL E32K Escherichia coli
    Tags: none
    Expression: Bacterial
    Mutation: E32K mutation conferring domi...
    Use:
    Deposited by: Csaba Pál
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