Search Results 1–5 of 5

Plasmids

1. PB-TAC-OSKM (Plasmid #80481)

   Purpose piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

   Insert: OSKM Mouse

   Tags: None

   Expression: Mammalian

   Mutation: None

   Use: piggyBac transposon

   Deposited by: Knut Woltjen
2. FUW-OSKM (Plasmid #20328)

   Purpose Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

   Insert: Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc Mouse

   Tags: None

   Expression: Mammalian

   Mutation: None

   Use: Lentiviral

   Deposited by: Rudolf Jaenisch
3. TetO-FUW-OSKM (Plasmid #20321)

   Purpose Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

   Insert: Oct-4 ( Pou5f1 Sox2 Klf4 Myc Mouse )

   Tags: None

   Expression: Mammalian

   Mutation: None

   Use: Lentiviral

   Deposited by: Rudolf Jaenisch
4. TetO-dCas9-D3A (Plasmid #78254)

   Purpose Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

   Insert: DNMT3A CD aa 598-912 Human

   Tags: Flag epitope tag

   Expression: Mammalian

   Mutation: None

   Use: CRISPR

   Deposited by: Grant Challen
5. TetO-dCas9-mD3A (Plasmid #78255)

   Purpose Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

   Insert: DNMT3A CD aa 598-912 ( DNMT3A Human )

   Tags: Flag epitope tag

   Expression: Mammalian

   Mutation: E756A

   Use: CRISPR

   Deposited by: Grant Challen