We narrowed to 9,662 results for: try

1. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Use

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR221-mCherry

   Plasmid

   #79509

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) mCherry RFP CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   mCherry RFP
   

   Use

   Multisite gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   no Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-PGK-destination

   Plasmid

   #60436

   Purpose

   Destination vector with PGK - Hygromycin selection marker

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. MXS_PGK::ZeoR-bGHpA

   Plasmid

   #62442

   Purpose

   MXS_chaining vector with PGK::ZeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Zeocin with PGK Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. psPax2-D64V-NC-mNeon

   Plasmid

   #196509

   Purpose

   mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   mNeon
   

   Use

   Tags

   mNeon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTagRFP657-C1

   Plasmid

   #31872

   Purpose

   For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

   Depositor

   Vladislav Verkhusha

   Article

   Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-mCherry-flex-dtA

   Plasmid

   #58536

   Purpose

   Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

   Depositor

   Naoshige Uchida

   Article

   Wu et al Nature. 2014 May 15;509(7500):325-30. do

   Insert

   dtA (DIP_RS18250 )
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1/hChR2(H134R)-EYFP

   Plasmid

   #20940

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. 460 p3E-2A-mCherrypA

   Plasmid

   #195970

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-mCherry
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. (+)-Limonene Synthase

    Plasmid

    #112230

    Purpose

    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

    Depositor

    Daniel Oprian

    Article

    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

    Insert

    (+)-Limonene Synthase
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-52

    Promoter

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAtetO_6xHis-Klentaq

    Plasmid

    #157862

    Purpose

    Plasmid for the aTc-inducible expression of the Klentaq

    Depositor

    Andrew Ellington

    Article

    Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

    Insert

    Klentaq
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgRNA_expression_vector

    Plasmid

    #210212

    Purpose

    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

    Depositor

    Albert Jeltsch

    Article

    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. opto-Rac1

    Plasmid

    #135396

    Purpose

    Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3

    Insert

    opto-Rac1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ES-FUCCI

    Plasmid

    #62451

    Purpose

    SMX_DEST vector with 3 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
    minPGK::Cherry-hCdt1(30-120)-bGHpA
    PGK::HygroR-bGpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. RNAPII_CTD27-52

    Plasmid

    #98678

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPEG

    Plasmid

    #162466

    Purpose

    Entry vector for gRNA insertion in plant prime editing expression vector

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Type

    Empty backbone

    Use

    Entry vector for grna

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pME-mKate2-P2A-TurboID-NS

    Plasmid

    #166568

    Purpose

    Multisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYN

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    mKate2-P2A-TurboID
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-CYLD-WT

    Plasmid

    #15506

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    CYLD (CYLD Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK017 pDEST exorh:EGFP

    Plasmid

    #195983

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIDTsmart-RC2-WT

    Plasmid

    #240057

    Purpose

    Express AAV2 WT capsid

    Depositor

    Abhishek Chatterjee

    Article

    Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

    Insert

    AAV2-WT Rep Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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