165,839 results

1. AAVS1-Bxb1-LP-v2-TC

   Plasmid

   #194326

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

   Insert

   loxP-EBFP2-attP-BleoR-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

   Plasmid

   #223747

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Lambda-PPase
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGK-T7/2-CD44s (fl)

   Plasmid

   #137812

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44s (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length, I186T and I253V on CD44

   Promoter

   PGK

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

   Plasmid

   #119816

   Purpose

   3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

   Depositor

   Ethan Abel

   Article

   Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

   Inserts

   Luciferase
   Puromycin
   

   Use

   Lentiviral and Luciferase

   Tags

   EGFP and V5

   Expression

   Mammalian

   Mutation

   Stop codon removed in the frame of the V5 frame v…

   Promoter

   CMV/TO and Human Phosphoglycerate kinase

   Available Since

   Jan. 2, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEGFP-N1-1x

   Plasmid

   #172281

   Purpose

   Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

   Depositor

   Antony K Chen

   Article

   Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

   Insert

   EGFP-N1-1x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABE8e-Cas9NG

   Plasmid

   #203328

   Purpose

   Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   ABE8e-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CLYBL-Bxb1-LP-v2-TC

   Plasmid

   #194327

   Purpose

   CLYBL targeting construct with the Bxb1 landing pad version 2 cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

   Insert

   loxP-EBFP2-attP-BleoR-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TO-hNGN2

   Plasmid

   #172115

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSGNluc

   Plasmid

   #186705

   Purpose

   A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

   Depositor

   Maria Leptin, Baubak Bajoghli

   Article

   Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

   Inserts

   GFP
   luciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiV_Neo_LKB1

    Plasmid

    #108111

    Purpose

    Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    LKB1 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP SNAP47

    Plasmid

    #101915

    Purpose

    Expresses SNAP47 in mammalian cells

    Depositor

    Thierry Galli

    Article

    Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

    Insert

    SNAP47 (SNAP47 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid

    #105550

    Purpose

    AAV expression of Cre from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB and AAV5

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PH-PLCD1-GFP

    Plasmid

    #51407

    Purpose

    Is a biosensor for PI(4,5)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPolI_SARS-CoV-2_NLuc

    Plasmid

    #183953

    Purpose

    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

    Depositor

    AJ te Velthuis

    Article

    Vial et al bioRxiv 2021.06.28.450211

    Insert

    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mammalian Pol I

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-n1-APP

    Plasmid

    #69924

    Purpose

    mammalian expression of human APP fused to EGFP

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    APP (APP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-fDIO-Cre (AAV1)

    Viral Prep

    #121675-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Tags

    None

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

17. pInducer20-Blast

    Plasmid

    #109334

    Purpose

    pInducer20 with added blasticidin resistance

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    Lentiviral; Inducible expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCMV_Bless

    Plasmid

    #207255

    Purpose

    Mammalian expression of Baboon Endogenous Retrovirus env protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    BaboonEndogenousRetrovirus_Rless_env
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

    Plasmid

    #118593

    Purpose

    Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha/TRE3G

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-HA-Ubiquitin-K48

    Plasmid

    #17605

    Purpose

    Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48 only, other lysines mutated to arginines. Enh…

    Promoter

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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