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Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae.
Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H
ACS Synth Biol. 2014 Sep 19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
60619pCRcrRNA expression plasmid on pRS423 backbone
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60620pCTPlasmid encoding iCas9 and tracrRNA on pRS415 backbone
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60621pCRCTPlasmid encoding iCas9, tracrRNA and crRNAs
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