Purpose(Empty Backbone) crRNA expression plasmid on pRS423 backbone
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60619||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5000
Vector typeYeast Expression, CRISPR
- Promoter SNR52
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
A portion of this plasmid was derived from a plasmid made byDr. Feng Zhang
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCR was a gift from Huimin Zhao (Addgene plasmid # 60619 ; http://n2t.net/addgene:60619 ; RRID:Addgene_60619)
For your References section:Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae. Bao Z, Xiao H, Liang J, Zhang L, Xiong X, Sun N, Si T, Zhao H. ACS Synth Biol. 2014 Sep 19. 10.1021/sb500255k PubMed 25207793