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N-Myc-53BP1 WT pLPC-Puro
(Plasmid #19836)


Item Catalog # Description Quantity Price (USD)
Plasmid 19836 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Scott Lowe
  • Backbone size w/o insert (bp) 5600
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    30C Stbl3
  • Copy number
    High Copy


  • Gene/Insert name
    p53 Binding Protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer TAGGCGTGTACGGTGGGA
  • 3′ sequencing primer CTTGCCAAACCTACAGGT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There may be a ~1.5kb band on a gel. This band is likely the result of a recombination product because of
the size of the construct. The maxipreps
probably contain two plasmids -- the full length and a short version that has
still retained the amp resistance gene but lost part of the construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N-Myc-53BP1 WT pLPC-Puro was a gift from Titia de Lange (Addgene plasmid # 19836 ; ; RRID:Addgene_19836)
  • For your References section:

    53BP1 promotes non-homologous end joining of telomeres by increasing chromatin mobility. Dimitrova N, Chen YC, Spector DL, de Lange T. Nature. 2008 Oct 19. ():. 10.1038/nature07433 PubMed 18931659