164,102 results

1. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPCX-PECAMTL

   Plasmid

   #45851

   Depositor

   Martin Schwartz

   Article

   Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

   Insert

   PECAM1 (PECAM1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV1)

   Viral Prep

   #87306-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Promoter

   EF1a

   Tags

   None

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pCAG-Cre

   Plasmid

   #13775

   Purpose

   Mammalian expression of Cre recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre
   

   Use

   Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.3-SARS2-B.1.617.2

   Plasmid

   #172320

   Purpose

   expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

   Depositor

   David Nemazee

   Article

   Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

   Insert

   Spike of B.1.617.2 strain (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

   Promoter

   CMV-F

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBR15.2-2H12.D11opt-scarRef

   Plasmid

   #221150

   Purpose

   cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2.1
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2.1)

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLAMP1-emiRFP670

   Plasmid

   #136570

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   LAMP1-emiRFP670
   

   Use

   Tags

   emiRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-mCherry (AAV1)

   Viral Prep

   #50459-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

9. Dazl-CPG-TV

   Plasmid

   #70144

   Purpose

   a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

   Depositor

   Rudolf Jaenisch

   Article

   Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

   Insert

   5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
   

   Use

   Mouse Targeting and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #168997

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-KIF5B*-strep

    Plasmid

    #120170

    Purpose

    Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Use

    Tags

    HA and Streptavidin

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV-U6+27-Tornado-F30-Squash-Broccoli

    Plasmid

    #177918

    Purpose

    Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Squash-Broccoli
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRGFP

    Plasmid

    #26475

    Purpose

    In vivo homologous recombination substrate composed of two differentially mutated GFP genes oriented as direct repeats and separated by a drug selection marker

    Depositor

    Maria Jasin

    Article

    Pierce et al Genes Dev. 1999 Oct 15. 13(20):2633-8.

    Insert

    DRGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. LD-GA

    Plasmid

    #209862

    Purpose

    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP A
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCE-mp53DD

    Plasmid

    #41856

    Purpose

    Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CAG

    Available since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-puro-EWS-FLI1

    Plasmid

    #102813

    Purpose

    Overexpress EWS-FLI1. 3rd generation lentiviral vector.

    Depositor

    Jialiang Wang

    Article

    Loganathan et al Oncotarget. 2016 Jul 12;7(28):43504-4351

    Insert

    EWS-FLI1 (EWSR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-cdc20

    Plasmid

    #11594

    Depositor

    Marc Kirschner

    Article

    Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

    Insert

    cdc20 (CDC20 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMCP-RecT

    Plasmid

    #183827

    Purpose

    pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    MCP-RecT_NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV_3XHA-ATP6V1B2

    Plasmid

    #228864

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Circular-oc-CVB3-GFP

    Plasmid

    #226262

    Purpose

    Production of in vitro circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge