165,839 results

1. MEK1-DD-V5

   Plasmid

   #217298

   Purpose

   Lentivirus vector for expression of MEK1 S218D/S222D

   Depositor

   Channing Der

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   S218D/S222D

   Promoter

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-2strep-FBH1

   Plasmid

   #236464

   Purpose

   transient overexpression of FBH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Jeong et al J Cell Biol. 2013 Jan 21;200(2):141-9. d

   Insert

   FBH1 (FBXO18 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available Since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAnc80L65AAP

   Plasmid

   #92307

   Purpose

   Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

   Depositor

   Luk Vandenberghe

   Article

   Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

   Insert

   Ancestral AAV Capsid Anc80L65AAP
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Rep2

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-G-Flamp1

   Plasmid

   #188567

   Purpose

   green biosensor for cAMP in mammalian cells

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

   Plasmid

   #213969

   Purpose

   AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   Shank3 sgRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. S2060

   Bacterial Strain

   #105064

   Purpose

   host strain for phage assisted continuous evolution

   Depositor

   David Liu

   Article

   Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

   Bacterial Resistance

   Streptomycin and tetracycline

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

8. GPI-RpHLuorin2

   Plasmid

   #171721

   Purpose

   GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to GPI anchor
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-IRES-Thy1.1 DEST

   Plasmid

   #17442

   Purpose

   Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

   Depositor

   Anjana Rao

   Article

   Wu et al Cell. 2006 Jul 28. 126(2):375-87.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-DIO-BACE-HA

    Plasmid

    #232353

    Purpose

    Beta-Secretase to be expressed (1:1) with either ATLAS protein.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Insert

    BACE (BACE1 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-TXNIP

    Plasmid

    #18758

    Depositor

    Clark Distelhorst

    Article

    Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

    Insert

    thioredoxin-interacting protein (Txnip Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-Bcl2

    Plasmid

    #18003

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK-flag-USP19

    Plasmid

    #78597

    Purpose

    Express flag-USP19 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. VPS13A^Halo

    Plasmid

    #118759

    Purpose

    VPS13A internally tagged with Halo at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Use

    Tags

    Halo tag inserted at position 1372

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. OTR untagged

    Plasmid

    #208913

    Purpose

    encodes the human oxytocin receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    OXTR (OXTR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TM5: 5x56, A218, Threonin instead of Alanin

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV rtTA3 Blast (w756-1)

    Plasmid

    #26429

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Blast

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)

    Plasmid

    #70741

    Purpose

    1 color strong reporter used in study

    Depositor

    Richard Murray

    Article

    Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

    Insert

    sfGFP-ssrA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. STK11 gRNA (BRDN0001146880)

    Plasmid

    #75912

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-Halo,IRES-eGFP

    Plasmid

    #164519

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

    Plasmid

    #221899

    Purpose

    Expresses alpha tubulin fused to mStayGold

    Depositor

    Janelia Integrative Imaging

    Insert

    mStayGold-aTubulin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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