Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Stat3 pcDNA3
(Plasmid #8706)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 8706 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone manufacturer
    Identical to pRc/CMV except at its MCS
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stat3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Mutation
    * see comments
  • GenBank ID
    U06922
  • Entrez Gene
    Stat3 (a.k.a. 1110034C02Rik, AW109958, Aprf)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Diagnostic restriction digest with XmaI (1.6kb and 6.1kb).

* E16K mutation found during QC. This variant is found in other published versions of this protein; should function as described in associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat3 pcDNA3 was a gift from Jim Darnell (Addgene plasmid # 8706 ; http://n2t.net/addgene:8706 ; RRID:Addgene_8706)
  • For your References section:

    Stat3 and Stat4: members of the family of signal transducers and activators of transcription. Zhong Z, Wen Z, Darnell JE Jr. Proc Natl Acad Sci U S A 1994 May 24;91(11):4806-10. 10.1073/pnas.91.11.4806 PubMed 7545930