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Addgene

Stat3 pcDNA3
(Plasmid #8706)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8706 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone manufacturer
    Identical to pRc/CMV except at its MCS
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Stat3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • Mutation
    * see comments
  • GenBank ID
    U06922
  • Entrez Gene
    Stat3 (a.k.a. 1110034C02Rik, Aprf)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Diagnostic restriction digest with XmaI (1.6kb and 6.1kb).

* E16K mutation found during QC. This variant is found in other published versions of this protein; should function as described in associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Stat3 pcDNA3 was a gift from Jim Darnell (Addgene plasmid # 8706 ; http://n2t.net/addgene:8706 ; RRID:Addgene_8706)
  • For your References section:

    Stat3 and Stat4: members of the family of signal transducers and activators of transcription. Zhong Z, Wen Z, Darnell JE Jr. Proc Natl Acad Sci U S A 1994 May 24;91(11):4806-10. 10.1073/pnas.91.11.4806 PubMed 7545930