|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27632||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6100
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameAMPK α1(1‐312)
SpeciesR. norvegicus (rat)
Insert Size (bp)2400
MutationConstitutively active by truncation after amino acid 312.
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer EF-1a Forward (Common Sequencing Primers)
This plasmid was first described in the following article: Crute et al. Functional domains of the alpha1 catalytic subunit of the AMP-activated protein kinase. J Biol Chem (1998) vol. 273 (52) pp. 35347-54.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEBG‐AMPK α1(1‐312) was a gift from Reuben Shaw (Addgene plasmid # 27632 ; http://n2t.net/addgene:27632 ; RRID:Addgene_27632)
For your References section:Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy. Egan DF, Shackelford DB, Mihaylova MM, Gelino SR, Kohnz RA, Mair W, Vasquez DS, Joshi A, Gwinn DM, Taylor R, Asara JM, Fitzpatrick J, Dillin A, Viollet B, Kundu M, Hansen M, Shaw RJ. Science. 2010 Dec 23. ():. 10.1126/science.1196371 PubMed 21205641