We narrowed to 15,620 results for: pET

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pET23a-His-hRanGAP1tail

Plasmid

#53139

Purpose

E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

RanGAP1 (RANGAP1 Human)

Tags

His

Expression

Bacterial

Mutation

C-terminal fragment (amino acids 398-587)

Promoter

T7

Available Since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b T7pCONS sfGFP

Plasmid

#154467

Purpose

pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Article

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET16b-MYPT1(1-314)

Plasmid

#224893

Purpose

Bacterial expression of MYPT1(1-314)

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

myosin phosphatase targeting subunit 1 (PPP1R12A )

Expression

Bacterial

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_05

Plasmid

#194666

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-SpyCatcher002-MBP

Plasmid

#216295

Purpose

Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SpyCatcher002-MBP

Tags

His6 tag and TEV cleavage site

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-GFP-AURKA

Plasmid

#99875

Purpose

Expression of GFP-AURKA for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Tags

GFP

Expression

Bacterial

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Gate2 ccdB

Plasmid

#117116

Purpose

Destination vector

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Type

Empty backbone

Use

Backbone for golden gate assembly for gene target…

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-30

Plasmid

#172754

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-30

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_01

Plasmid

#194662

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_01 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-C2m2-mKate

Plasmid

#208765

Purpose

To express C2m2-mKate in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2m2-mKate (Mfge8 Mouse)

Tags

mKate

Expression

Bacterial

Mutation

Mutated 24 and 45 lysine to asparagine in C2 doma…

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-His6

Plasmid

#51532

Purpose

Express His6-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pET3a-His-DJ1

Plasmid

#51488

Purpose

Inducible expression of human His-DJ1 in E.coli

Depositor

Michael J Fox Foundation MJFF

Insert

DJ1 (PARK7 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET23-CBD-(-PP)-EGFP

Plasmid

#12597

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

Cdc42 Biosensor (WAS Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

F271C

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pETM-11-6His-DmBelle_B

Plasmid

#145950

Purpose

Bacterial Expression of DmBelle

Depositor

Elisa Izaurralde

Insert

DmBelle (bel Fly)

Expression

Bacterial

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-18

Plasmid

#172763

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-18

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP

Plasmid

#185013

Purpose

Bacterial Expression of 6xHis Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

Sirius-GFP

Expression

Bacterial

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a-trAtCCD8(pYL735)

Plasmid

#178286

Purpose

Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET26-Ufd1-His

Plasmid

#21266

Depositor

Hemmo Meyer

Article

Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

Insert

Ufd1 (Ufd1l Mouse)

Tags

His

Expression

Bacterial

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pET28b-CoilR-Cys

Plasmid

#181989

Purpose

Used to label proteins with the CoilE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

CoilR-Cys

Tags

His-Tag

Expression

Bacterial

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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