A signaling inspired synthetic toolkit for efficient production of tyrosine phosphorylated proteins.
Ryan MM, Portelance R, Newman GF, Martinez G, Shekharan S, Wu A, Angel S, Schaberg KE, Gilmore P, Sprung R, Townsend R, Naegle KM
bioRxiv [Preprint]. 2025 Feb 18:2024.12.22.629992. doi: 10.1101/2024.12.22.629992.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 259379 | kVh-ABL | Kinase co-expression for phosphorylation of substrate |
| 259380 | kVh-EPHB1 | Kinase co-expression for phosphorylation of substrate |
| 259382 | kVh-FES | Kinase co-expression for phosphorylation of substrate |
| 259383 | kVh-FGFR3 | Kinase co-expression for phosphorylation of substrate |
| 259384 | kVh-MERTK | Kinase co-expression for phosphorylation of substrate |
| 259385 | kVh-LYN | Kinase co-expression for phosphorylation of substrate |
| 259386 | kVh-EPHA4 | Kinase co-expression for phosphorylation of substrate |
| 259388 | sVd-EGFRCtail | Substrate co-expression for phosphorylation |
| 259389 | sVdW-EGFRCtail | Substrate co-expression for phosphorylation |
| 259390 | sVdAvi-GAB1 | Substrate co-expression for phosphorylation |
| 259391 | sVdAviW-GAB1 | Substrate co-expression for phosphorylation |
| 259393 | pETHG-PD1_Y223 | Substrate co-expression for phosphorylation |
| 259394 | pETSF-PD1_Y248 | Substrate co-expression for phosphorylation |
| 259395 | pETHF-PD1_Y223 | Substrate co-expression for phosphorylation |
| 259396 | pETSG-PD1_Y248 | Substrate co-expression for phosphorylation |
| 259397 | sVf-PTPN11_NSH2 | Substrate co-expression for phosphorylation |
| 259398 | sVfAvi-PTPN11_NSH2 | Substrate co-expression for phosphorylation |
| 259399 | sVfAviWW-PTPN11_NSH2 | Substrate co-expression for phosphorylation |
| 259400 | sVdAlfa-PTPN11_NSH2 | Substrate co-expression for phosphorylation |