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sVdpep-EGFR_ Y1172
(Plasmid #259392)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 259392 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    sVd (pGEX mod)
  • Backbone manufacturer
    Sigma Aldrich
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR Y1172 21mer
  • Species
    H. sapiens (human)
  • Tags / Fusion Proteins
    • msmt3-p40W-GlyLinker (N terminal on backbone)
    • mutatedGST-Myc-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SacI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.22.629992 for bioRxiv preprint.

Please note that Addgene's sequencing results differ from the full sequence information provided by the depositing laboratory, finding A32T in lacI CDS feature compared to the depositor reference. The depositor confirms that this change is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sVdpep-EGFR_ Y1172 was a gift from Kristen Naegle (Addgene plasmid # 259392 ; http://n2t.net/addgene:259392 ; RRID:Addgene_259392)

  • For your References section:

    A signaling inspired synthetic toolkit for efficient production of tyrosine phosphorylated proteins. Ryan MM, Portelance R, Newman GF, Martinez G, Shekharan S, Wu A, Angel S, Schaberg KE, Gilmore P, Sprung R, Townsend R, Naegle KM. bioRxiv [Preprint]. 2025 Feb 18:2024.12.22.629992. doi: 10.1101/2024.12.22.629992. 10.1101/2024.12.22.629992 PubMed 39763866
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