kVh-SRC
(Plasmid
#259387)
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PurposeKinase co-expression for phosphorylation of substrate
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 259387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonekVh (modified pBAD)
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Backbone manufacturerThermoFisher
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSRC
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SpeciesH. sapiens (human)
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Entrez GeneSRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
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Tags
/ Fusion Proteins
- smt3-SH3 (N terminal on backbone)
- MYC (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRobert Lefkowitz, pcDNA3 c-SRC (WT) (Addgene Plasmid #42202)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.22.629992 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
kVh-SRC was a gift from Kristen Naegle (Addgene plasmid # 259387)