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pmOrange-Fb(GFP)

Plasmid

#249295

Purpose

pmOrange-Fb(GFP) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Methods. 2026 Apr 22. doi: 10.1038/s

Insert

pmOrange-Fb(GFP)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-Fb(GFP)

Plasmid

#249296

Purpose

pLSSmOrange-Fb(GFP) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Methods. 2026 Apr 22. doi: 10.1038/s

Insert

pLSSmOrange-Fb(GFP)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_hPTK7-gRNA2

Plasmid

#249197

Purpose

human PTK7 CRISPR KO

Depositor

Weihong Tan

Article

Guoyan Luo et et al Science391,eadv6127(2026)

Insert

PTK7 (PTK7 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pDD1773 pCS2-PMMVp

Plasmid

#251797

Purpose

Potyviral protease specific against heptapeptide sequence: EPMKFQV

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

PMMVp

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-AtTIR1P10A-Myc-MBP-HA

Plasmid

#228943

Purpose

Expression AtTIR1P0A-Myc and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtTIR1P10A (TIR1 Mustard Weed)

Tags

myc

Expression

Bacterial

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-hCDCP1-gRNA3

Plasmid

#249218

Purpose

CDCP1 CRISPR KO

Depositor

Weihong Tan

Article

Guoyan Luo et et al Science391,eadv6127(2026)

Insert

CDCP1 (CDCP1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Feb. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-hCDCP1-gRNA2

Plasmid

#249217

Purpose

CDCP1 CRISPR KO

Depositor

Weihong Tan

Article

Guoyan Luo et et al Science391,eadv6127(2026)

Insert

CDCP1 (CDCP1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Feb. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-hCDCP1-gRNA1

Plasmid

#249216

Purpose

CDCP1 CRISPR KO

Depositor

Weihong Tan

Article

Guoyan Luo et et al Science391,eadv6127(2026)

Insert

CDCP1 (CDCP1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Feb. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_(3'UTR)_RLEloop

Plasmid

#240253

Purpose

PROTEUS vector with no transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pJW53(uTEV3-DHFR2.0-IRES-mCherry)

Plasmid

#248363

Purpose

Lentiviral vector that can express destabilized uTEV3. Expressed off of CMV promoter with mCherry marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

uTEV3-DHFR2.0

Use

Lentiviral

Promoter

CMV

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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VN-dCasE-VC-GK

Plasmid

#164277

Purpose

Express VN-dCasE-VC protein in mammalian cells. This plasmid is also referred to as as VN-dEcCas6-VC-GK in the publication.

Depositor

Chunyu Han

Article

Gao et al Nucleic Acids Res. 2022 May 6;50(8):e46.

Insert

VN-dCasE-VC

Expression

Mammalian

Mutation

H20A in EcCasE

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iC

Plasmid

#231418

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pJAT22

Plasmid

#204311

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-GFPn

Use

CRISPR

Promoter

3XP3

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-U6tetO_shFF4

Plasmid

#229207

Purpose

lvl 1 expresion vector

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

Pol3 expresion of shFF4

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iA

Plasmid

#231417

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-AtUBC8-S

Plasmid

#222713

Purpose

Expression AtUBC8-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtUBC8 (UBC8 Mustard Weed)

Tags

S-Tag

Expression

Bacterial

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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lvl1-Adapter1-SapI

Plasmid

#229200

Purpose

lvl 1 adapter

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

T2-T4 - Sap1 adapapter

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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