We narrowed to 266 results for: T7 rna polymerase

1. pHT-PRD

   Plasmid

   #87755

   Purpose

   Bacterial expression vector for producing 6x histidine tagged dynamin 1a proline rich domain (residues 746-864)

   Depositor

   Yu-Wen Hwang

   Insert

   Semi-synthetic gene coding for dynamin 1a proline rich domain (residues 746-864)
   

   Tags

   6X histidine tag

   Expression

   Bacterial

   Promoter

   T7 RNA polymerase promoter

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS1

   Plasmid

   #55749

   Purpose

   Produces aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. T444T

   Plasmid

   #113081

   Purpose

   Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. T777T

   Plasmid

   #113082

   Purpose

   Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.

   Depositor

   Tibor Vellai

   Article

   Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

   Type

   Empty backbone

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGV3319

   Plasmid

   #170277

   Purpose

   Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   Bst-LF D720A
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   D720A

   Promoter

   T7-lac

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-DEST42-dre4

   Plasmid

   #170440

   Purpose

   Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   dre4 (dre4 Fly)
   

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-20

   Promoter

   T7

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS4

   Plasmid

   #55751

   Purpose

   Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 1-59 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS5

   Plasmid

   #55750

   Purpose

   Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 59-264 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. rFIXX-bp chain A (IRESneo)

   Plasmid

   #82450

   Purpose

   Chain A of the habu snake venom FIX/FX binding protein with a C-terminal polyhistidine-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring neomycin resistance.

   Depositor

   Robert Peters

   Insert

   rFIXX-bp chain A
   

   Tags

   Polyhistidine

   Expression

   Bacterial and Mammalian

   Promoter

   T7 RNA polymerase promoter

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. rFIXX-bp chain B (IRESpuro)

    Plasmid

    #82451

    Purpose

    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.

    Depositor

    Robert Peters

    Insert

    rFIXX-bp chain B
    

    Tags

    Biotin

    Expression

    Bacterial and Mammalian

    Promoter

    T7 RNA polymerase promoter

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2,0_3M5M_eGFP

    Plasmid

    #135474

    Purpose

    Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, eGFP reporter
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2,0_3M5M_luciferase

    Plasmid

    #135475

    Purpose

    Encodes Marburg virus minigenome with luciferase reporter gene under control of a T7 RNA polymerase promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    Marburg virus minigenome, luciferase reporter
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    T7

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJP_Azul01

    Plasmid

    #219670

    Purpose

    Blue light inducible system

    Depositor

    Yolanda Schaerli

    Article

    Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

    Inserts

    similar to T7 RNA polymerase (core)
    similar to YF1-fixJ
    

    Use

    Synthetic Biology

    Promoter

    LacIq35 and pJ23105

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfB8589

    Plasmid

    #175221

    Purpose

    Xenopus oocyte expression vector containing YMR253C. Contains T7 RNA polymerase binding site, and adds polyA tail to YMR253C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YMR253C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB8707

    Plasmid

    #175222

    Purpose

    Xenopus oocyte expression vector containing YNL095C. Contains T7 RNA polymerase binding site, and adds polyA tail to YNL095C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YNL095C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB8728

    Plasmid

    #175223

    Purpose

    Xenopus oocyte expression vector containing YOL060C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL060C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOL060C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB8709

    Plasmid

    #175224

    Purpose

    Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOL092W (CEN.PK strain)
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB8711

    Plasmid

    #175225

    Purpose

    Xenopus oocyte expression vector containing YOR092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOR092W
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB8713

    Plasmid

    #175226

    Purpose

    Xenopus oocyte expression vector containing YOR271C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR271C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOR271C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB8590

    Plasmid

    #175227

    Purpose

    Xenopus oocyte expression vector containing YOR307C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR307C. Used for generation of mRNA, for injection into xenopus laevis oocytes.

    Depositor

    Irina Borodina

    Article

    Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

    Insert

    YOR307C
    

    Use

    Mrna expression, injection into xenopus oocytes

    Tags

    PolyA

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge