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Timothy Whitehead Lab Plasmids


The Timothy Whitehead Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
The Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead TA PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014.
Removal and upgrading of lignocellulosic fermentation inhibitors by in situ biocatalysis and liquid-liquid extraction. Tomek Kyle J, Saldarriaga Carlos Rafael Castillo, Velasquez Fernando Peregrino Cordoba, Liu Tongjun, Hodge David B, Whitehead Timothy A Biotechnology and bioengineering Volume 112, Issue 3, pages 627–632, March 2015.
Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Stapleton JA, Whitehead TA, Nanda V Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836.
Nicking Mutagenesis Plasmids (unpublished)
Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning. Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 10.1073/pnas.1614437114.
Comprehensive sequence-flux mapping of a levoglucosan utilization pathway in E. coli. Klesmith JR, Bacik JP, Michalczyk R, Whitehead TA ACS Synth Biol. 2015 Sep 14.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
59836pJK_proB_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with proB promoter pET-29b(+) amiE PA3366 Add to Cart
59837pJK_proK17_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with proK17 promoter pET-29b(+) amiE PA3366 Add to Cart
59838pJK_proK16_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with proK16 promoter pET-29b(+) amiE PA3366 Add to Cart
59839pJK_proK14_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with proK14 promoter pET-29b(+) amiE PA3366 Add to Cart
59840pJK_proK9_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with proK9 promoter pET-29b(+) amiE PA3366 Add to Cart
59841pJK_proK6_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with proK6 promoter pET-29b(+) amiE PA3366 Add to Cart
59842pJK_proK3_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with proK3 promoter pET-29b(+) amiE PA3366 Add to Cart
59843pJK_proK1_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with proK1 promoter pET-29b(+) amiE PA3366 Add to Cart
59844pJK_pro6_amiEALIPHATIC AMIDASE (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses AmiE with pro6 promoter pET-29b(+) amiE PA3366 Add to Cart
59845pJK_proB_eGFPEnhanced Green Fluorescent Protein (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses eGFP with proB promoter pET-29b(+) Add to Cart
59847pJK_proK16_eGFPEnhanced Green Fluorescent Protein (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses eGFP with proK16 promoter pET-29b(+) Add to Cart
59848pJK_proK14_eGFPEnhanced Green Fluorescent Protein (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses eGFP with proK14 promoter pET-29b(+) Add to Cart
59849pJK_proK9_eGFPEnhanced Green Fluorescent Protein (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses eGFP with proK9 promoter pET-29b(+) Add to Cart
59850pJK_proK6_eGFPEnhanced Green Fluorescent Protein (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses eGFP with proK6 promoter pET-29b(+) Add to Cart
59851pJK_pro6_eGFPEnhanced Green Fluorescent Protein (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses eGFP with pro6 promoter pET-29b(+) Add to Cart
59852pJK_proK3_eGFPEnhanced Green Fluorescent Protein (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses eGFP with proK3 promoter pET-29b(+) Add to Cart
59853pJK_proK1_eGFPEnhanced Green Fluorescent Protein (Other)Bacterial ExpressionThe Interrelationship between Promoter Strength, Gene Expression, and Growth Rate. PLoS One. 2014 Oct 6;9(10):e109105. doi: 10.1371/journal.pone.0109105. eCollection 2014. Constitutively expresses eGFP with proK1 promoter pET-29b(+) Add to Cart
60630pJK_kanR_proJK1_PADPhenolic acid decarboxylase padCBacterial ExpressionRemoval and upgrading of lignocellulosic fermentation inhibitors by in situ biocatalysis and liquid-liquid extraction. Biotechnology and bioengineering Volume 112, Issue 3, pages 627–632, March 2015. Constitutively expresses PAD (phenolic acid decarboxylase) pET-29b(+) padC BSU34400 Add to Cart
62639pJexpress414_OmpA171_R4consOmpA_R4cons (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 pJexpress Add to Cart
62646pJexpress414_OmpA171_WTOmpA (Other)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Wild-type OmpA (N-terminal domain) with an HA tag in the 4th external loop pJexpress414 Add to Cart
62647pJexpress414_OmpA171_H1346OmpA_H1346 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1346 pJexpress414 Add to Cart
62648pJexpress414_OmpA171_H1256OmpA_H1256 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1256 pJexpress414 Add to Cart
62649pJexpress414_OmpA171_H2345OmpA_H2345 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 2345 pJexpress414 Add to Cart
62650pJexpress414_OmpA171_H3567OmpA_H3567 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 3567 pJexpress414 Add to Cart
62651pJexpress414_OmpA171_H2368OmpA_H2368 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 2368 pJexpress414 Add to Cart
62652pJexpress414_OmpA171_H1678OmpA_H1678 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1678 pJexpress414 Add to Cart
62653pJexpress414_OmpA171_WTnoGirdlesOmpA_noGirdles (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Wild-type OmpA with aromatic girdle residues replaced by leucine pJexpress414 Add to Cart
64290pJexpress414_OmpA171_H1237OmpA_H1237 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1237 pJexpress414 Add to Cart
64291pJexpress414_OmpA171_H2578OmpA_H2578 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 2578 pJexpress414 Add to Cart
64292pJexpress414_OmpA171_H3478OmpA_H3478 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 3478 pJexpress414 Add to Cart
64293pJexpress414_OmpA171_H1248OmpA_H1248 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1248 pJexpress414 Add to Cart
64294pJexpress414_OmpA171_H2467OmpA_H2467 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 2467 pJexpress414 Add to Cart
64295pJexpress414_OmpA171_H1457OmpA_H1457 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1457 pJexpress414 Add to Cart
64296pJexpress414_OmpA171_H4568OmpA_H4568 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 4568 pJexpress414 Add to Cart
64297pJexpress414_OmpA171_H1358OmpA_H1358 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1358 pJexpress414 Add to Cart
64298pJexpress414_OmpA171_R4cons_G125LOmpA_R4cons_G125L (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with G125L mutation pJexpress Add to Cart
64299pJexpress414_OmpA171_R4cons_G41AOmpA_R4cons_G41A (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with G41A mutation pJexpress Add to Cart
64300pJexpress414_OmpA171_R4cons_G99IOmpA_R4cons_G99I (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with G99I mutation pJexpress Add to Cart
64301pJexpress414_OmpA171_R4cons_P121FOmpA_R4cons_P121F (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with P121F mutation pJexpress Add to Cart
64302pJexpress414_OmpA171_R4cons_Y129HOmpA_R4cons_Y129H (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. Conservative version of OmpA Redesign 4 with Y129H mutation pJexpress Add to Cart
64310pJexpress414_OmpA171_H-3OmpA_H-3 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 1245678 pJexpress414 Add to Cart
64311pJexpress414_OmpA171_H-46OmpA_H-46 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 123578 pJexpress414 Add to Cart
64312pJexpress414_OmpA171_H346OmpA_H346 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 346 pJexpress414 Add to Cart
64313pJexpress414_OmpA171_H-78OmpA_H-78 (Synthetic)Bacterial Expression, Synthetic BiologyComputational redesign of the lipid-facing surface of the outer membrane protein OmpA. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. OmpA redesign hybrid with wild-type strands 123456 pJexpress414 Add to Cart
80085pEDA5_GFPmut3_Y66HGFPmut3_Y66H (Synthetic)Bacterial Expression Nicking Mutagenesis Plasmids (unpublished) Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol. pET29b(+) Add to Cart
81160pJK_proJK1_LGKLevoglucosan kinase wild-type (Synthetic)Bacterial ExpressionComprehensive sequence-flux mapping of a levoglucosan utilization pathway in E. coli. ACS Synth Biol. 2015 Sep 14. Expresses levoglucosan kinase with promoter proJK1 pJK Add to Cart
81161pJK_proNR2_LGK.1Levoglucosan kinase (Synthetic)Bacterial ExpressionComprehensive sequence-flux mapping of a levoglucosan utilization pathway in E. coli. ACS Synth Biol. 2015 Sep 14. Expresses levoglucosan kinase variant LGK.1 with promoter proNR2 pJK Add to Cart
81162pJK_proNR2_LGK.9Levoglucosan kinase (Synthetic)Bacterial ExpressionComprehensive sequence-flux mapping of a levoglucosan utilization pathway in E. coli. ACS Synth Biol. 2015 Sep 14. Expresses levoglucosan kinase variant LGK.9 with promoter proNR2 pJK Add to Cart
81163pSALECTNK-TEM1(S70A,D179G)/csTEM1TEM-1 beta-lactamase (Synthetic)Bacterial ExpressionTrade-offs between enzyme fitness and solubility illuminated by deep mutational scanning. Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 10.1073/pnas.1614437114. pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1 pSALECT Add to Cart
81164pSALECTNK-LGK/csTEM1Levoglucosan kinase (Synthetic)Bacterial ExpressionTrade-offs between enzyme fitness and solubility illuminated by deep mutational scanning. Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 10.1073/pnas.1614437114. pSALECT plasmid with levoglucosan kinase fused to TEM-1 pSALECT Add to Cart
81165pETconNK-LGKLevoglucosan kinase wild-type (Synthetic)Bacterial Expression, Yeast ExpressionTrade-offs between enzyme fitness and solubility illuminated by deep mutational scanning. Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 10.1073/pnas.1614437114. pETconNK with levoglucosan kinase pETconNK Add to Cart
81166pETconNK-TEM1(S70A,D179G)TEM-1 beta-lactamase (Synthetic)Bacterial Expression, Yeast ExpressionTrade-offs between enzyme fitness and solubility illuminated by deep mutational scanning. Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 10.1073/pnas.1614437114. pETconNK with TEM1(S70A,D179G) beta-lactamase pETconNK Add to Cart
81169pETconNKBacterial Expression, Yeast ExpressionTrade-offs between enzyme fitness and solubility illuminated by deep mutational scanning. Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 10.1073/pnas.1614437114. pETconNK Empty Backbone pETconNK
  • Tag / Fusion Protein
    • c-myc (C terminal on backbone)
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