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DT033A

Plasmid

#159394

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Publication

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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BC-A1-008

Plasmid

#78695

Purpose

A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.

Depositor

Brian Chow

Publication

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator

Use

Synthetic Biology

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBW100ParsR

Plasmid

#78635

Purpose

pSB3K3 carrying J101-rbs32-arsR-t-ParsR

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J101-rbs32-arsR-t-ParsR

Use

Synthetic Biology

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBW205J105-gfp

Plasmid

#78644

Purpose

pSB3K3 carrying J105-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J105-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW203J116-gfp

Plasmid

#78642

Purpose

pSB3K3 carrying J116-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J116-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pBW202J114-gfp

Plasmid

#78641

Purpose

pSB3K3 carrying J114-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J114-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
MXS_loxP

Plasmid

#62430

Purpose

MXS_chaining vector with loxP

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

loxP site

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pX458-ITGB6

Plasmid

#235244

Purpose

Encodes gRNA for human ITGB6

Depositor

Jan Carette

Publication

Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

Insert

ITGB6 (ITGB6 Human)

Use

CRISPR

Expression

Mammalian

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-MYADM

Plasmid

#235245

Purpose

Encodes gRNA for human MYADM

Depositor

Jan Carette

Publication

Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

Insert

MYADM (MYADM Human)

Use

CRISPR

Expression

Mammalian

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-7

Plasmid

#228989

Purpose

For bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-9

Plasmid

#228968

Purpose

For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-12

Plasmid

#228970

Purpose

For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-3

Plasmid

#228963

Purpose

For bacterial expression of anti-GFP nanobody LaG94-3, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-3

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-38

Plasmid

#228979

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-38

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-8

Plasmid

#228967

Purpose

For bacterial expression of anti-GFP nanobody LaG94-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-6

Plasmid

#228966

Purpose

For bacterial expression of anti-GFP nanobody LaG94-6, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-6

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-18

Plasmid

#228972

Purpose

For bacterial expression of anti-GFP nanobody LaG94-18, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-18

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-2

Plasmid

#228975

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaGIgG-4

Plasmid

#228998

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-4, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-2

Plasmid

#228962

Purpose

For bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Publication

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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