We narrowed to 15,156 results for: pET

1. 6his R.3-AR GUSpET28a+/pLysS

   Plasmid

   #61157

   Purpose

   N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

   Depositor

   Andrew Ellington, Ichiro Matsumura

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   GUSAR
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

   Promoter

   T7

   Available Since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11d-FHis-DND1

   Plasmid

   #70083

   Purpose

   Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein. Codons are optimized for bacteria expression.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Tags

   6xHIS and FLAG

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET20b-delta133HtrA2 S400A

   Plasmid

   #16155

   Depositor

   L. Miguel Martins

   Article

   Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

   Insert

   HtrA2 (HTRA2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   S400A

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pET20b-CeNLuc-6xHis

   Plasmid

   #135933

   Purpose

   Expresses the CeNLuc LumiFluor in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   CeNLuc LumiFluor
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-Drp1(deltaIB)

   Plasmid

   #73041

   Purpose

   Bacterial expression of mouse Drp1 isoform b (minus residues 514-602) with C-terminal His-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Drp1(deltaIB) (Dnm1l Mouse)
   

   Tags

   6xHis-tag and PreScission Protease site

   Expression

   Bacterial

   Mutation

   Deleted amino acids 514-602

   Promoter

   T7

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET11a+D77R/T214V

   Plasmid

   #66135

   Purpose

   Expresses the D77R/T214V mutant of CYP119

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Expression

   Bacterial

   Mutation

   D77R, T214V

   Promoter

   T7 promoter

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. HM3C NaVSp1_p pET24b

   Plasmid

   #127852

   Purpose

   Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaVSp1
   

   Tags

   3C Cleavage site, His Tag, and MBP

   Expression

   Bacterial

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet-msGC-NT13

   Plasmid

   #75087

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 1-380 of the beta subunit and fragment 4…

   Available Since

   May 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-Hook3 aa 1-160

    Plasmid

    #74607

    Purpose

    Expresses Hook domain of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-160 (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_0385_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1

    Plasmid

    #116997

    Purpose

    Expresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Rivularia sp. PCC 7116 RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-21a(+)-LpxA

    Plasmid

    #12684

    Depositor

    SeWon Suh

    Article

    Lee et al Proteins. 2003 Nov 15. 53(3):772-4.

    Insert

    UDP-N-acetylglucosamine acyltransferase (HP1375 Helicobacter pylori)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28a-CylA

    Plasmid

    #48198

    Purpose

    Expresses CylA (fatty acyl-AMP ligase) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylA
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-PesaR-Dlux

    Plasmid

    #47657

    Purpose

    PesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    PesaR-D
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Added an additional esa box upstream of -35 site …

    Promoter

    PesaR-D

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pET26-CNTN149S/F366A

    Plasmid

    #100165

    Purpose

    recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

    Depositor

    Seok-Yong Lee

    Article

    Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

    Insert

    Concentrative nucleoside transporter
    

    Tags

    MBP-His

    Expression

    Bacterial

    Mutation

    N149S, F366A

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b-H74A-PvuRts1I

    Plasmid

    #86894

    Purpose

    express H74A variant PvuRts1I in ER2566 strain

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    PvuRts1I (pvuRts1I )
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    W205A

    Promoter

    T7

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b-hTIG3-1-164

    Plasmid

    #100724

    Purpose

    Full-length human TIG3 in E.coli expression vector

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    RARRES3 (PLAAT4 Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promotor

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-p53 (Dog 1-77)

    Plasmid

    #62060

    Purpose

    Expression of Dog p53 (residues 1-77) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-77

    Promoter

    T7

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET3a pro-RegIIIg

    Plasmid

    #64934

    Purpose

    bacterial expression of full length mouse RegIIIgamma

    Depositor

    Lora Hooper

    Article

    Cash et al Protein Expr Purif. 2006 Jul;48(1):151-9

    Insert

    RegIIIg (Reg3g Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pETM6-5F5-mCherry

    Plasmid

    #73422

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 5F5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P5F5 (orthogonal T7-lac variant)

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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