We narrowed to 5,844 results for: pCas

1. pCAG-MBP-ATG9 (692-839)

   Plasmid

   #188092

   Purpose

   To make monomeric ATG9 C-terminal tail for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (692-839)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-MBP-ATG9 (801-839)

   Plasmid

   #188093

   Purpose

   To make monomeric ATG9 C-terminal tail for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (801-839)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-OSF-ATG13 (2-197)-K18D

   Plasmid

   #188096

   Purpose

   Expresses human ATG13 HORMA K18D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-K18D

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-OSF-ATG13 (2-197)-K15D

   Plasmid

   #188094

   Purpose

   Expresses human ATG13 HORMA K15D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-OSF-ATG13 (2-197)-W50D

   Plasmid

   #188098

   Purpose

   Expresses human ATG13 HORMA W50D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-W50D

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-OSF-ATG13 (2-197)-Y152D

   Plasmid

   #188103

   Purpose

   Expresses human ATG13 HORMA Y152D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-Y152D

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-OSF-ATG13 (2-197)-R153D

   Plasmid

   #188104

   Purpose

   Expresses human ATG13 HORMA R153D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-R153D

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-OSF-ATG13 (2-197)-F16D

   Plasmid

   #188095

   Purpose

   Expresses human ATG13 HORMA F16D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-F16D

   Promoter

   CMV

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-OSF-ATG13 (2-197)-Y118D

   Plasmid

   #188101

   Purpose

   Expresses human ATG13 HORMA Y118D mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)-Y118D

   Promoter

   CMV

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-OSF-ATG13 (2-197)-E83L

    Plasmid

    #188099

    Purpose

    Expresses human ATG13 HORMA E83L mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-E83L

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-OSF-ATG13 (2-197)-Y115D

    Plasmid

    #188100

    Purpose

    Expresses human ATG13 HORMA Y115D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-Y115D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-OSF-ATG13 (2-197)-F19D

    Plasmid

    #188097

    Purpose

    Expresses human ATG13 HORMA F19D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-F19D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-GST-ATG101-W110D/F112D

    Plasmid

    #188106

    Purpose

    Expresses human ATG101 HORMA W110D/F112D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG101 (ATG101 Human)
    

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    codon-optimized ATG101-W110D/F112D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-OSF-ATG13 (2-197)-L151D

    Plasmid

    #188102

    Purpose

    Expresses human ATG13 HORMA L151D mutant in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    strep-strep-Flag tag

    Expression

    Mammalian

    Mutation

    ATG13 (2-197)-L151D

    Promoter

    CMV

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

    Plasmid

    #178854

    Purpose

    Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)
    

    Expression

    Mammalian

    Mutation

    Grb2: Y160E

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

    Plasmid

    #178857

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

    Plasmid

    #178858

    Purpose

    Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJCC_162 SpCas9 KES(1107-1109)GG

    Plasmid

    #179526

    Purpose

    For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 KES(1107-1109)GG
    

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    replaced KES (residues 1107-1109) with GG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

    Plasmid

    #176236

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176237

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge