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We narrowed to 5,415 results for: golden gate

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Showing: 2341 - 2360 of 5415 results

  4. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-487610

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  5. PX458 V3

    Plasmid
    #226957
    Purpose
    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226957-plasmid-map-sequence-id-450777

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  7. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Assembly of Pumilio and FBF (PUF) homology domain based RNA-binding proteins for recognition of RNA targets.
    Depositor
    Huimin Zhao
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Bacterial Expression, Mammalian Expression
    Editing Type
    PUF
    Cloning Type
    Golden Gate
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. PDG458 V3

    Plasmid
    #226959
    Purpose
    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226959-plasmid-map-sequence-id-450819

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  12. pECNUS4

    Plasmid
    #184887
    Purpose
    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery
    Depositor
    Eunyoung Chae
    Article
    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    RPS5A
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    184887-plasmid-map-sequence-id-437896

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  13. CIDAR MoClo Extension, Volume I

    Plasmid Kit
    #1000000161
    Purpose
    Plasmids to efficiently build a wide variety of gene expression constructs. Can be used as a stand-alone toolkit or with the CIDAR MoClo Parts Kit (Addgene #1000000059).
    Depositor
    Richard Murray
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only

  16. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    Collection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  19. Fungal Toolkit for Modular Cloning (FTK)

    Plasmid Kit
    #1000000191
    Purpose
    Synthetic biology entry vectors with ready-to-use fungal genetic parts for rapid construction of genetic circuits as well as CRISPR components for efficient genome engineering in filamentous fungi.
    Depositor
    Arnold Driessen
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Fungal Expression
    Editing Type
    CRISPR
    Cloning Type
    Golden Gate
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only

  20. pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]

    Plasmid
    #247262
    Purpose
    Express ISCro4 recombinase and bridge RNA
    Depositor
    Patrick Hsu
    Article
    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1
    Insert
    pNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
    Expression
    Mammalian
    Mutation
    C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    247262-plasmid-map-sequence-id-490480

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Showing: 2341 - 2360 of 5415 results