We narrowed to 3,795 results for: golden gate

1. pAS1_4x7SKPylT_EF1_Ub-BsmBI

   Plasmid

   #162801

   Purpose

   STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPOR1CB0113

   Plasmid

   #117552

   Purpose

   Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
   

   Expression

   Plant

   Promoter

   2x35S+5'UTR OMEGA (pICH51288)

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCALNL-BsaI-EGFP

   Plasmid

   #123136

   Purpose

   Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   EGFP, mRFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   cBA

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGC001

   Plasmid

   #48824

   Purpose

   Provides WUS as GreenGate coding sequence module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   WUS (WUS Mustard Weed)
   

   Use

   Golden gate compatible cloning vector

   Mutation

   internal BsaI recognition site removed by silent …

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT195

   Plasmid

   #127843

   Purpose

   clone BE4max CBEs by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   BE4max without deaminase
   

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTU2_2a

   Plasmid

   #73010

   Purpose

   Secondary module (BpiI) site in pTU2a backbone

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Secondary module + Golden Gate pTU2a BsmBI destination site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUS_A8

   Plasmid

   #99882

   Purpose

   Recipient vector for Golden Gate TALE cloning system. Used to assemble 8 TALE repeats.

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   TALEN

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPOR0SP0011

   Plasmid

   #117530

   Purpose

   Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-xCas9 3.7 (no stop codon)
   

   Use

   CRISPR

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEV749

   Plasmid

   #246450

   Purpose

   The plasmid comprises an indicible transcriptional unit encoding EYFP

   Depositor

   Ron Weiss

   Article

   Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

   Insert

   EYFP
   

   Use

   Synthetic Biology

   Promoter

   miniYB

   Available Since

   Oct. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBA1540

    Plasmid

    #246061

    Purpose

    Bacterial expression of 2TMbeta protein under the pJex promoter

    Depositor

    Jennifer Doudna

    Article

    Doherty et al bioRxiv [Preprint]. 2025 Apr 1:2025.03.2

    Insert

    2TMbeta
    

    Expression

    Bacterial

    Promoter

    pJEX promoter

    Available Since

    Oct. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP3613 [Cloning backbone, recombinase]

    Plasmid

    #247264

    Purpose

    Cloning backbone for cloning bridge recombinase

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKG1179_pPV1-EFS

    Plasmid

    #239690

    Purpose

    BsaI Golden Gate assembly plasmid encoding the EFS promoter

    Depositor

    Kate Galloway

    Article

    Peterman et al Nucleic Acids Research, Volume 53, Issue

    Insert

    EFS promoter
    

    Use

    Synthetic Biology

    Available Since

    Oct. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. p0AmpCD-TXdact

    Plasmid

    #237273

    Purpose

    Carries the X. dendrorhous act terminator

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    TXdact
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p0AmpCD-TXdgpd

    Plasmid

    #237272

    Purpose

    Carries the X. dendrorhous gdh terminator

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    TXdgpd
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. p0AmpAB-PXdgdh

    Plasmid

    #237269

    Purpose

    Carries the X. dendrorhous gdh promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdgdh
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. p0AmpAB-PXdadh4

    Plasmid

    #237268

    Purpose

    Carries the X. dendrorhous adh4 promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdadh4
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. p0AmpAB-PXdact

    Plasmid

    #237267

    Purpose

    Carries the X. dendrorhous act promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdact
    

    Use

    Synthetic Biology

    Available Since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLP-PrhaBAD-pcepA-himar-chlorR

    Plasmid

    #238492

    Purpose

    Landing Pad with RhaRS/PrhaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposase

    Depositor

    Farren Isaacs

    Article

    Nakamura et al ACS Synth Biol. 2025 Mar 21;14(3):943-95

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    RhaRS/PrhaBAD

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p0AmpAB-PXdUspA

    Plasmid

    #237241

    Purpose

    Carries the X. dendrorhous UspA promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdUspA
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. p0AmpAB-PXdGrg1

    Plasmid

    #237242

    Purpose

    Carries the X. dendrorhous Grg1 promoter

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Insert

    PXdGrg1
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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