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We narrowed to 5,692 results for: golden gate

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Showing: 2341 - 2360 of 5692 results

  1. 4G-Cloning Plasmid Kit

    Plasmid kit
    #1000000252
    Purpose
    Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.
    Depositor
    Stephan Gruber
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  2. pGGG Wheat GUS marker

    Plasmid
    #165418
    Purpose
    Wheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Publication
    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.
    Insert
    Os Ubiquitin Promoter::Gus (2x introns)::NosT
    Use
    Wheat transformation binary vector
    Promoter
    OS UbiP::GUS (2xint)::NosT
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165418-plasmid-map-sequence-id-331269

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  3. pNK497

    Plasmid
    #219754
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Publication
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Expression
    Plant
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219754-plasmid-map-sequence-id-458646

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  7. Fragmid

    Plasmid kit
    #1000000237
    Purpose
    Toolkit for rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.
    Depositor
    John Doench
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    AAV, Insect Expression, Lentiviral, Mammalian Expression
    Editing type
    CRISPR
    Cloning type
    Golden Gate (MoClo)
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

    Plasmid
    #247160
    Purpose
    Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Puromycin resistance, mCherry
    Expression
    Mammalian
    Promoter
    Ef1a
    Available since
    Nov. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    247160-plasmid-map-sequence-id-492681

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  11. Start-Stop Assembly Toolkit

    Plasmid kit
    #1000000152
    Purpose
    Modular, scarless DNA assembly method suitable for combinatorial assembly and ideal for metabolic engineering.
    Depositor
    John Heap
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only

  13. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    IIya Finkelstein
    Publication
    Buffington et al Nat Biotechnol. 2025 Oct 23. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CAG, H1, and U6
    Available since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    237445-plasmid-map-sequence-id-476973

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  19. Komagataeibacter Tool Kit (KTK)

    Plasmid kit
    #1000000216
    Purpose
    A collection of standardized parts used for hierarchical assembly of single and multi-gene constructs for Komagataeibacter species.
    Depositor
    Tom Ellis
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 2341 - 2360 of 5692 results