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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS L5-L2

Plasmid

#62085

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQCas12j2

Plasmid

#189780

Purpose

Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

Cas12j2

Use

CRISPR; Gateway compatible cas12j2 entry clone

Tags

NLS

Expression

Plant

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV L1-R5

Plasmid

#62090

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 M

Plasmid

#158369

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 M (M )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR221-PGK.Pro

Plasmid

#79489

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

PGK Promoter (PGK1 Synthetic, Human)

Use

Multisite gateway entry clone

Promoter

PGK

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP14

Plasmid

#158365

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP14 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-SMAD3

Plasmid

#79507

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

SMAD3 (SMAD3 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP12

Plasmid

#158363

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP12 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP11

Plasmid

#158362

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP11 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMX-mTAF12

Plasmid

#44342

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF12 (Taf12 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 L5-L2

Plasmid

#62095

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR mCherry L5-L2

Plasmid

#62184

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing mCherry. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

mCherry

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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plexm-mouse LRRC33

Plasmid

#111601

Purpose

Express mouse LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

mouse LRRC33 (Nrros Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Starts at aa 18

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS L5-L4

Plasmid

#62089

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pET30-hDlg PDZ2

Plasmid

#21692

Depositor

James Baleja

Article

Liu et al Biochemistry. 2007 Sep 25. 46(38):10864-

Insert

hDlg/SAP97 PDZ2 domain (DLG1 Human)

Tags

His6

Expression

Bacterial

Mutation

human hDlg/SAP97 PDZ2 domain (residues 318-405)

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 Orf3a

Plasmid

#158372

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf3a (ORF3a )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEPQD2KN0897

Plasmid

#177090

Purpose

MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR eGFP L5-L2

Plasmid

#62183

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing EGFP. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

eGFP

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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