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pDONRG_P4-P1R:TBA2p

Plasmid

#128429

Purpose

Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.

Depositor

George Haughn

Publication

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Insert

TESTA ABUNDANT 2 (AT1G62060 Mustard Weed)

Use

Gateway promoter entry clone

Promoter

TESTA ABUNDANT 2

Available since

Oct. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

Plasmid

#69231

Purpose

Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 TALE

Use

CRISPR

Tags

2X NLS and NLS-3XHA-NLS

Expression

Mammalian

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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1287 p3E-mut let-7 etv2 3’ UTR

Plasmid

#49011

Purpose

Zebrafish Long length etv2 3' UTR with five let7 binding site mutations in gateway 3' Entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

Mutated the Let7 miRNA binding sites

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only
GFP-GW1D1aMut(W>A)

Plasmid

#21522

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa254-503, mutate every tryptophan (total…

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pLX304-BCL-XL-F97W

Plasmid

#203572

Purpose

Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

Depositor

Abhishek Chakraborty

Publication

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

Changed Phenylalanine 97 to Tryptophan for partia…

Promoter

CMV

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

Plasmid

#194753

Purpose

Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Publication

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-BioID2-HA-13 x Linker- RBP(V)-GPI-PSS

Use

Lentiviral

Tags

HA

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194752

Purpose

Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Publication

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-RBP(V)-13 x Linker-BioID2-HA-GPI-PSS

Use

Lentiviral

Tags

HA

Promoter

CMV

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2EFMUT

Plasmid

#174333

Purpose

encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Publication

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

NKD2132DFD134>AFA (substitutions of D132 and D…

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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ORF18(W170A_G171A)-3xFLAG pCDNA4

Plasmid

#129815

Purpose

Expresses KSHV ORF18 (W170A_G171A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Publication

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Tryptophan 170 to Alanine and Glycine 171…

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi

Plasmid

#89744

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Publication

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET30Xa-SBL1(160E,NoCys)

Plasmid

#66875

Purpose

Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

Depositor

Betty Gaffney

Publication

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations

Tags

Histag

Expression

Bacterial

Mutation

S160E; changed 4 (all) cysteines to serine

Promoter

T7lac

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-dLIS1

Plasmid

#47047

Purpose

Expression of tagged Drosophila LIS1

Depositor

Laura Lee

Publication

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Tags

Myc

Expression

Mammalian

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
CS2-CHY-dLIS1

Plasmid

#47045

Purpose

Expression of fluorescent Drosophila LIS1

Depositor

Laura Lee

Publication

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Lis1 (Lis-1 Fly)

Tags

CHY

Expression

Mammalian

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only
pCMV6 CHIP delTPR-Myc

Plasmid

#246737

Purpose

Mammalian expression of human CHIP with TPR domain deleted, and a myc-tag on its C-terminus

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

deletion of TPR domains

Promoter

CMV

Available since

May 21, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1425TAG)-HA

Plasmid

#239763

Purpose

Expresses mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

K1425TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Nav1.6(K1546TAG)-HA

Plasmid

#239764

Purpose

Expresses mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

K1546TAG

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-FLAG-HAND1-T2A-mTagBFP2

Plasmid

#223192

Purpose

Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

Depositor

Matthew Birket

Publication

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Promoterless entry vector for gateway cloning

Tags

FLAG and T2A-mTagBFP2

Expression

Bacterial

Promoter

None

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP35

Plasmid

#196773

Purpose

Contains RCD1 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

RADICAL-INDUCED CELL DEATH1 (RCD1 Mustard Weed)

Use

Greengate (gg) entry vector

Mutation

248 G->C

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PIP07

Plasmid

#196745

Purpose

Contains CERK1 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

CHITIN ELICITOR RECEPTOR KINASE 1 (CERK1 Mustard Weed)

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR207-MUM2sp-ADPG2

Plasmid

#170723

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

Depositor

George Haughn

Publication

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)

Use

Gateway donor vector / entry clone

Available since

Nov. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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