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p3E-GBP

Plasmid

#67672

Purpose

Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

anti-GFP nanobody

Use

Gateway multisite 3' entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR223-BRRF2

Plasmid

#195820

Purpose

Gateway Entry Clone of Epstein-Barr virus BRRF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BRRF2 (BRRF2 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BXLF1

Plasmid

#195825

Purpose

Gateway Entry Clone of Epstein-Barr virus BXLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BXLF1 (BXLF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BFRF3

Plasmid

#195778

Purpose

Gateway Entry Clone of Epstein-Barr virus BFRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BFRF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BHRF1

Plasmid

#195780

Purpose

Gateway Entry Clone of Epstein-Barr virus BHRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BHRF1 (BHRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RV

Plasmid

#188544

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RV

Use

CRISPR; Gateway compatible lbcas12a-rv entry clone

Tags

NLS

Expression

Plant

Mutation

G146R, R182V

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

Plasmid

#127360

Purpose

gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS L1-R5

Plasmid

#62084

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR223-BKRF3

Plasmid

#195790

Purpose

Gateway Entry Clone of Epstein-Barr virus BKRF3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BKRF3

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF2

Plasmid

#195800

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf7b

Plasmid

#158376

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7b (ORF7b )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194750

Purpose

To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP15

Plasmid

#158366

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP15 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

Plasmid

#127357

Purpose

gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNLF2a

Plasmid

#195767

Purpose

Gateway Entry Clone of Epstein-Barr virus BNLF2a

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNLF2a (BNLF2a Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BNLF2b

Plasmid

#195770

Purpose

Gateway Entry Clone of Epstein-Barr virus BNLF2b

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNLF2b (BNLF2b Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP7

Plasmid

#158358

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP7 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221 Cas9-RFC5

Plasmid

#183200

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC5

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6-miR-30 L1-R5

Plasmid

#62113

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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