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JN402: pMVP (R4-R3) Firefly Luciferase

Plasmid

#121741

Purpose

pMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Firefly Luciferase

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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PIGP_pCSdest

Plasmid

#53827

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PIGP (PIGP Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pX459V2.0+K848A

Plasmid

#108296

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMIG small T C97S E102Q

Plasmid

#9060

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 97, 102 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

C97S, E102Q

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
pAG416GPD-Cerulean-CHC1

Plasmid

#18850

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

CHC1 (CHC1 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
Lenti-sgNeo3/Cre

Plasmid

#89653

Purpose

Expresses a Neomycin-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Neomycin

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos Y345A

Plasmid

#8970

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Y345A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
Lenti-sgSetd2#2/Cre

Plasmid

#89650

Purpose

Expresses a Setd2-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting Setd2 (Setd2 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAN1935

Plasmid

#127203

Purpose

Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1

Use

Synthetic Biology

Available Since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMIG small T 110

Plasmid

#9061

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 110 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

contains only the N-termial 110 amino acids

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
TFF1_pCSdest

Plasmid

#53908

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TFF1 (TFF1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
IG101: pMVP (L5-L4) TRE promoter

Plasmid

#121729

Purpose

pMVP L5-L4 entry plasmid, contains TRE promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos F343A

Plasmid

#8969

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

F343A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
MIS18A_pCSdest

Plasmid

#53821

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MIS18A (MIS18A Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
MTK234_041

Plasmid

#123916

Purpose

Encodes the spCas9 mScarlet gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Smp_090100-Cd4-bio-His

Plasmid

#120655

Purpose

Expression of recombinant S. mansoni protein

Depositor

Gavin Wright

Article

Cécile et al bioRXiv

Insert

Smp_090100 (Smp_090100 Schistosoma mansoni)

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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Smp_112090-Cd4-bio-His

Plasmid

#120662

Purpose

Expression of recombinant S. mansoni protein

Depositor

Gavin Wright

Article

Cécile et al bioRXiv

Insert

Smp_112090 (Smp_112090 Schistosoma mansoni)

Tags

ratCd4(d3+4)-bio-6His

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFoxd3IRESGFPtv

Plasmid

#37266

Depositor

Patricia Labosky

Article

Hanna et al Genes Dev. 2002 Oct 15;16(20):2650-61.

Insert

Foxd3 (Foxd3 Mouse)

Use

Mouse Targeting

Tags

IRES-GFP

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
KU701: pMVP (L5-L4) eGFP

Plasmid

#121707

Purpose

pMVP L5-L4 entry plasmid, contains eGFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eGFP fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eGFP (open)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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PSMG1_pCSdest

Plasmid

#53884

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PSMG1 (PSMG1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits