We narrowed to 45,481 results for: crispr

1. pDonor_mU6

   Plasmid

   #69350

   Purpose

   sgRNA scaffold and mouse U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   mU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Promoter

   mU6 promoter

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

   Plasmid

   #99698

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV

   Depositor

   George Church

   Type

   Empty backbone

   Use

   AAV

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EC64434

   Plasmid

   #209518

   Purpose

   Cloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)

   Depositor

   Wendy Harwood, Cristobal Uauy

   Article

   Lawrenson et al Research Square, 12 March 2024, PREPRINT

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6_gRNA_handle_U6t

   Plasmid

   #49016

   Purpose

   gRNA expression empty vector (mammalian cells)

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRPR1_gRNA_handle_RPR1t

   Plasmid

   #49014

   Purpose

   gRNA expression empty vector (yeast)

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   pRPR1

   Available Since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+hSpCas9

   Plasmid

   #51815

   Purpose

   Encode hSpCas9 for in vitro transcription with Sp6

   Depositor

   Masato Kinoshita

   Article

   Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

   Insert

   humanized S.pyogenes Cas9
   

   Use

   CRISPR; Medaka, zebrafish

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDonor_hU6

   Plasmid

   #69312

   Purpose

   sgRNA scaffold and human U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   hU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Promoter

   human U6 promoter

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pV1025

   Plasmid

   #111424

   Purpose

   Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   CaCas9
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWUKI 1+2

   Plasmid

   #79879

   Purpose

   Encodes Cas1+2 and a CRISPR array

   Depositor

   George Church

   Article

   Shipman et al Science. 2016 Jun 9. pii: aaf1175.

   Inserts

   Cas1
   Cas2
   CRISPR Array
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMLS338

    Plasmid

    #73719

    Purpose

    SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Unc-32::GFP(Cbr-unc-119)
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCD185

    Plasmid

    #113317

    Purpose

    dCas9, MCP-SoxS_R93A

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9 and MCP-SoxS_R93A
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    R93A

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPN10-gCTG

    Plasmid

    #114385

    Purpose

    pPN10 with gRNA for spCRISPR-Cas9 targeted to CTG repeat, PAM: CTG

    Depositor

    Vincent Dion

    Article

    Cinesi et al Nat Commun. 2016 Nov 9;7:13272. doi: 10.

    Insert

    gCTG
    

    Use

    CRISPR

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA with U6 promoter

    Plasmid

    #48962

    Purpose

    to drive the sgRNA expression under a U6 promoter

    Depositor

    Mario de Bono

    Article

    Chen et al Nucleic Acids Res. 2013 Sep 5.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7tyrgRNA

    Plasmid

    #46761

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    tyr gRNA (tyr Zebrafish)
    

    Use

    CRISPR

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH41308::hCas9

    Plasmid

    #49770

    Purpose

    Level 0 hCas9 module

    Depositor

    Sophien Kamoun

    Article

    Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

    Insert

    hCas9
    

    Use

    CRISPR

    Available Since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB4279

    Plasmid

    #98698

    Purpose

    A control plasmid containing the intact bsdMX marker

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIK86

    Plasmid

    #47933

    Purpose

    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.

    Depositor

    Iskra Katic

    Article

    Katic et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR and Unspecified

    Promoter

    T7

    Available Since

    Oct. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPhygCAS9

    Plasmid

    #63555

    Purpose

    Expresses CAS9 in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    Humanized Streptococcus pyogenes Cas9 from PX330 (Addgene)
    

    Use

    Leishmania expression

    Tags

    flag

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pV1010

    Plasmid

    #111423

    Purpose

    gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    sgADE-NT2
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. hgRNA-A21_pLKO-Hyg

    Plasmid

    #100559

    Purpose

    hgRNA-A21 in a lentiviral backbone

    Depositor

    George Church

    Article

    Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

    Insert

    hgRNA-A21
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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