We narrowed to 302 results for: Weissman Jonathan

1. pLG1-puro-sgATL3-5

   Plasmid

   #109011

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL3-sgRNA #5 (ATL3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCH45 (multiAsCas12a-KRAB lenti)

   Plasmid

   #217330

   Purpose

   Lentiviral expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCH4 (denAsCas12a-KRAB lenti)

   Plasmid

   #217331

   Purpose

   Lentiviral expression of denAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   denAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Mutation

   D908A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLG1-puro-sgULK1-1

   Plasmid

   #109004

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ULK1-sgRNA #1 (ULK1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLG1-puro-sgATL2-1

   Plasmid

   #109008

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL2-sgRNA #1 (ATL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLG1-puro-sgATL2-2

   Plasmid

   #109009

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL2-sgRNA #2 (ATL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-hU6-cs1-PuroR-T2A-BFP

   Plasmid

   #230934

   Purpose

   gRNA cloning vector with direct capture sequence 1 in stem loop 2

   Depositor

   Charles Gersbach

   Article

   Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

   Pooled Library

   #197348

   Purpose

   To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

   Depositor

   Rebecca Voorhees, Jonathan Weissman

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

9. mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

   Pooled Library

   #197349

   Purpose

   To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

   Depositor

   Rebecca Voorhees, Jonathan Weissman

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

10. hCRISPRi_dual_1_2

    Pooled Library

    #187246

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

11. hCRISPRa_dual_1_2

    Pooled Library

    #187249

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

12. hCRISPRi_dual_5_6

    Pooled Library

    #187248

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

13. hCRISPRi_dual_3_4

    Pooled Library

    #187247

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

14. hCRISPRa_dual_3_4

    Pooled Library

    #187250

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

15. hCRISPRa_dual_5_6

    Pooled Library

    #187251

    Purpose

    Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

16. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-mir144~451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164793

    Purpose

    Express miR-144~451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144~451 (MIR451A, MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLG1-puro-sgATL1-5

    Plasmid

    #109007

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL1-sgRNA #5 (ATL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLG1-puro-sgATL1-2

    Plasmid

    #109006

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL1-sgRNA #2 (ATL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pU6-sgCtrl_EF1a-Puro-T2A-BFP

    Plasmid

    #195500

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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