We narrowed to 309 results for: Weissman Jonathan

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNAEmptyVector_Puro_T2A_BFP2

Plasmid

#236729

Purpose

Dual sgRNA empty vector expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Type

Empty backbone

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 1

Plasmid

#109002

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #1

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 3

Plasmid

#109003

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #3

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-2

Plasmid

#109010

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #2 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-5

Plasmid

#109011

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #5 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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hCRISPRi_dual_1_2

Pooled Library

#187246

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRa_dual_1_2

Pooled Library

#187249

Purpose

Ultra-compact, human genome-wide CRISPRa library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRi_dual_3_4

Pooled Library

#187247

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197348

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

Depositor

Rebecca Voorhees, Jonathan Weissman

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

Pooled Library

#197349

Purpose

To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

Depositor

Rebecca Voorhees, Jonathan Weissman

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Species

Homo sapiens

Use

Lentiviral

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only
hCRISPRi_dual_5_6

Pooled Library

#187248

Purpose

Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRa_dual_3_4

Pooled Library

#187250

Purpose

Ultra-compact, human genome-wide CRISPRa library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
hCRISPRa_dual_5_6

Pooled Library

#187251

Purpose

Ultra-compact, human genome-wide CRISPRa library targeting each gene with a single element encoding a dual sgRNA cassette.

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Species

Synthetic

Use

Lentiviral

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only
pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-cs1-PuroR-T2A-BFP

Plasmid

#230934

Purpose

gRNA cloning vector with direct capture sequence 1 in stem loop 2

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgULK1-1

Plasmid

#109004

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ULK1-sgRNA #1 (ULK1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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