We narrowed to 3,830 results for: Gateway entry

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pME-MCS (JDW 455)

Plasmid

#229832

Purpose

A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attL1/L2 flanked MCS

Use

Gateway Cloning

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPtGG-1

Plasmid

#89990

Purpose

pBR322-based entry vector for Gateway cloning

Depositor

Philip Weyman

Publication

Bielinski et al PeerJ Preprints. 2017 5:e2907v1

Type

Empty backbone

Use

Golden gate entry vector

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pGGEV_2_+nls-eGFP-HA-_+1_BK+

Plasmid

#49312

Purpose

Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

nls-EGFP-HA

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pME-loxP-3xSTOP-loxP (AJ30)

Plasmid

#257879

Purpose

Middle entry Gateway plasmid with LoxP flanked trancriptional stops to make conditional transgenics

Depositor

Abigail Jensen

Insert

loxP-flanked transcriptional STOP

Use

Cre/Lox and Gateway Cloning

Promoter

none

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Publication

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p221z-dCAS9p-t35s

Plasmid

#118387

Purpose

Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Publication

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

dCAS9p

Use

CRISPR

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-EGFP-P2A-iCre (JDW 921)

Plasmid

#224519

Purpose

A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP-P2A-iCre

Use

Cre/Lox

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

Plasmid

#224489

Purpose

Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Sun1-2xsfGFP-6xMYC-pA

Use

Gateway Cloning

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

Plasmid

#224501

Purpose

Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lamin B Vhh mNeonGreen-pA

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-GAP43-ECFP-stop-L2

Plasmid

#186356

Purpose

Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Publication

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Growth-associated protein-43 (Gap43 Synthetic, Mouse)

Use

Expression of a fluorescent membrane marker

Tags

ECFP

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGGEV_2_XcmI-LacZ

Plasmid

#49297

Purpose

Golden Gate entry vector at position 2, LacZ

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_3_Linker

Plasmid

#49283

Purpose

Golden Gate entry vector at position 3, small linker insert

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a (JDW 1164)

Plasmid

#224473

Purpose

Gateway compatible 5' entry clone containing the human EF1a promoter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EF1a promoter

Expression

Mammalian

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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p5E TRE-3GV (JDW 1163)

Plasmid

#224476

Purpose

Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TRE3GV promoter

Use

Gateway Cloning

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-hs_ICAM_short (JDW 1046)

Plasmid

#224480

Purpose

Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

min-ICAM2 promoter

Expression

Mammalian

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME EFS-rtTA/rtTA3 (JDW 1230)

Plasmid

#224525

Purpose

A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS-rtTA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME TAEL2.0-5xC120-minpro (JDW 1109)

Plasmid

#224507

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL2.0-5xC120

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ210

Plasmid

#109329

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

Depositor

Yiping Qi

Publication

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGGEV_+1_Linker

Plasmid

#49280

Purpose

Golden Gate entry vector at position 1,small linker insert, plus 5' ATG

Depositor

Joachim Wittbrodt

Publication

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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