DNA-binding-domain fusions enhance the targeting range and precision of Cas9.
Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu LJ, Wolfe SA
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. PubMed Article
Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu LJ, Wolfe SA
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69219 | pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268 | Expresses wild type SpCas9 fused to Zif268 in mammalian cells |
| 69220 | pCSDest2-SpCas9-NLS-3XHA-NLS | Expresses wild type SpCas9 in mammalian cells |
| 69221 | pCSDest2-SpCas9-MT1-NLS-3XHA-NLS | Expresses R1333K mutant SpCas9 in mammalian cell |
| 69222 | pCSDest2-SpCas9-MT2-NLS-3XHA-NLS | Expresses R1333S mutant SpCas9 in mammalian cell |
| 69223 | pCSDest2-SpCas9-MT3-NLS-3XHA-NLS | Expresses R1335K mutant SpCas9 in mammalian cell |
| 69224 | pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268 | Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell |
| 69225 | pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2 | Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells |
| 69226 | pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3 | Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells |
| 69227 | pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4 | Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells |
| 69228 | pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2 | Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell |
| 69229 | pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3 | Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell |
| 69230 | pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4 | Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell |
| 69231 | pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3 | Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell |
| 69232 | pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry | Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites) |
| 69233 | pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry | R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites) |
| 69234 | pLKO1-puro-U6-sgRNA-TS2 | U6 driven SpCas9 sgRNA expression for TS2 site |
| 69235 | pLKO1-puro-U6-sgRNA-TS3 | U6 driven SpCas9 sgRNA expression for TS3 site |
| 69236 | pLKO1-puro-U6-sgRNA-TS4 | U6 driven SpCas9 sgRNA expression for TS4 site |
| 69237 | pLKO1-puro-U6-sgRNA-LRTM2 | U6 driven SpCas9 sgRNA expression for LRTM2 site |
| 69238 | pLKO1-puro-U6-sgRNA-KANK3 | U6 driven SpCas9 sgRNA expression for KANK3 site |
| 69239 | pLKO1-puro-U6-sgRNA-TGM2 | U6 driven SpCas9 sgRNA expression for TGM2 site |
| 69240 | pLKO1-puro-U6-sgRNA-PLXNB2 | U6 driven SpCas9 sgRNA expression for PLXNB2 site |