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DNA-binding-domain fusions enhance the targeting range and precision of Cas9.

Bolukbasi MF, Gupta A, Oikemus S, Derr AG, Garber M, Brodsky MH, Zhu LJ, Wolfe SA
Nat Methods. 2015 Oct 19. doi: 10.1038/nmeth.3624. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
69219pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268Expresses wild type SpCas9 fused to Zif268 in mammalian cells
69220pCSDest2-SpCas9-NLS-3XHA-NLSExpresses wild type SpCas9 in mammalian cells
69221pCSDest2-SpCas9-MT1-NLS-3XHA-NLSExpresses R1333K mutant SpCas9 in mammalian cell
69222pCSDest2-SpCas9-MT2-NLS-3XHA-NLSExpresses R1333S mutant SpCas9 in mammalian cell
69223pCSDest2-SpCas9-MT3-NLS-3XHA-NLSExpresses R1335K mutant SpCas9 in mammalian cell
69224pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell
69225pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells
69226pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells
69227pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells
69228pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell
69229pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell
69230pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell
69231pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell
69232pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentryWild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
69233pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentryR1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
69234pLKO1-puro-U6-sgRNA-TS2U6 driven SpCas9 sgRNA expression for TS2 site
69235pLKO1-puro-U6-sgRNA-TS3U6 driven SpCas9 sgRNA expression for TS3 site
69236pLKO1-puro-U6-sgRNA-TS4U6 driven SpCas9 sgRNA expression for TS4 site
69237pLKO1-puro-U6-sgRNA-LRTM2U6 driven SpCas9 sgRNA expression for LRTM2 site
69238pLKO1-puro-U6-sgRNA-KANK3U6 driven SpCas9 sgRNA expression for KANK3 site
69239pLKO1-puro-U6-sgRNA-TGM2U6 driven SpCas9 sgRNA expression for TGM2 site
69240pLKO1-puro-U6-sgRNA-PLXNB2U6 driven SpCas9 sgRNA expression for PLXNB2 site

Antibodies from Article