We narrowed to 9,409 results for: try

1. pET51b-His-TEV-SNAP-tag_fast

   Plasmid

   #167271

   Purpose

   Overexpression of the fast variant (E30R) of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAPf-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   E30R

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR_L1-miniTurbo-YFP-NLS-STOP-L2

   Plasmid

   #127362

   Purpose

   gateway entry vector for expressing nuclear miniTurbo-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. KK601: pMVP (L3-L2) ecDHFR DD + polyA

   Plasmid

   #121790

   Purpose

   pMVP L3-L2 entry plasmid, contains ecDHFR DD + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stabilized by TMP) to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   ecDHFR Degron Domain + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

   Plasmid

   #139448

   Purpose

   Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag E2-crimson
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. NEK7_HUMAN_D0

   Plasmid

   #79735

   Purpose

   This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   NEK7 (NEK7 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pENTR-2B-Tbx5

   Plasmid

   #98619

   Purpose

   Gateway entry vector TBX5

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   TBX5 (Tbx5 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-mCREB

   Plasmid

   #68551

   Purpose

   AAV expression of mCREB

   Depositor

   Eric Nestler

   Article

   Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

   Insert

   mCREB
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S133A

   Promoter

   CMV

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pENTR_L1-Turbo-NES-YFP-STOP-L2

   Plasmid

   #127358

   Purpose

   gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Myc-CYLD-C601A

   Plasmid

   #60027

   Purpose

   Myc-tagged CYLD catalytic inactive (C601A) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. ND4 right TALE–G1333 DddAtox C

    Plasmid

    #158095

    Purpose

    expresses the right-side ND4 TALE–DddAtox half in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-C–1xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. ND4 left TALE–G1333 DddAtox N

    Plasmid

    #158093

    Purpose

    expresses the left-side ND4 TALE–DddAtox half in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-N–1xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR4_ELAVL1

    Plasmid

    #106103

    Purpose

    Entry vector for ELAVL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    ELAVL1 (ELAVL1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. G1397 DddAtox-C–SaKKH-Cas9(D10A)

    Plasmid

    #157840

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSaPIPLC His

    Plasmid

    #173810

    Purpose

    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87

    Insert

    Staphylococcus aureus plc
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR1A mKate2-hu_ncOGT WT

    Plasmid

    #154282

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Expression

    Mutation

    WT OGT

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2xFlag CMV2-YAP2-1st&2nd WW mutant

    Plasmid

    #19048

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine,and Proline 202…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ230-RRVQ

    Plasmid

    #200188

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V, E795Q

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-YFP-DEPTOR

    Plasmid

    #73390

    Purpose

    Expresses HA and YFP tagged DEPTOR

    Depositor

    Jie Chen, Taekjip Ha

    Article

    Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

    Insert

    DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)
    

    Use

    Tags

    HA and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pMX-hTAF11

    Plasmid

    #44341

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF11 (TAF11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMX_hTAF5

    Plasmid

    #44336

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF5 (TAF5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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