We narrowed to 3,293 results for: phage

1. Pvalb-2A-Dre Flp-in replacement vector

   Plasmid

   #61571

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb-2A-Dre (Pvalb D6 Bacteriophage, Mouse)
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. ptdTHC

   Plasmid

   #112617

   Purpose

   promoterless expression plasmid driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-tdTomato (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Promoterless vector, ready for promoter for mamma…

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   No Promoter

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BE3(E63Q)-P2A-EGFP (pRZ189)

   Plasmid

   #123613

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(E63Q)-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   E63Q in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVHC

   Plasmid

   #112614

   Purpose

   promoterless expression plasmid driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Venus (H2BC3 Aequorea victoria, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Promoterless vector, ready for promoter for mamma…

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   No Promoter

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. nCas9-UGI control for Target-AID (pRZ1047)

   Plasmid

   #131299

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-EHC

   Plasmid

   #112619

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Emerald (H2BC3 Aequorea victoria, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEHC

   Plasmid

   #112615

   Purpose

   promoterless expression plasmid driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Emerald (H2BC3 Aequorea victoria, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Promoterless vector, ready for promoter for mamma…

   Tags

   Emerald

   Expression

   Mammalian

   Promoter

   No Promoter

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmTHC

   Plasmid

   #112616

   Purpose

   promoterless expression plasmid driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-mTFP1 (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Promoterless vector, ready for promoter for mamma…

   Tags

   mTeal (mTFP1)

   Expression

   Mammalian

   Promoter

   No Promoter

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 18065-M04-412

   Plasmid

   #225667

   Purpose

   Lentivirus protein expression. Control plasmid.

   Depositor

   Dominic Esposito

   Article

   Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

   Inserts

   dsmCherry
   delCD19 (CD19 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Mutation

   C-terminally truncated (aa 1-333 only)

   Promoter

   WPRE-SV40p and mCMVp

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmTHC_PGKneoLox2DTA.2

    Plasmid

    #112624

    Purpose

    H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-mTFP1 (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    mTeal (mTFP1)

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEHC_PGKneoLox2DTA.2

    Plasmid

    #112623

    Purpose

    H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Emerald

    Promoter

    No Promoter

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. 18065-M01-412

    Plasmid

    #225664

    Purpose

    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

    Depositor

    Dominic Esposito

    Article

    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

    Inserts

    dsCopGFP
    delCD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Mutation

    C-terminally truncated (aa 1-333 only)

    Promoter

    SORE6-mCMVp and WPRE-SV40p

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-Flag-ER-Hoxb8-IRES-GFP

    Plasmid

    #222292

    Purpose

    Less commonly used plasmid for generating ER-Hoxb8 conditionally immortalized myeloid cell lines.

    Depositor

    Mark Kamps, David Sykes

    Article

    Wang et al Nat Methods. 2006 Apr;3(4):287-93.

    Insert

    ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    The estrogen receptor hormone binding domain cont…

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCag SE (Self-excising) FlpO-2A-Cre EV

    Plasmid

    #130986

    Purpose

    episomal expression of FlpO and Cre recombinases, self excising

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    FlpO-2A-Cre
    

    Use

    Cre/Lox and Unspecified; Episomal expression vect…

    Expression

    Mammalian

    Mutation

    protamine intron added to FlpO to prevent bacteri…

    Promoter

    CMV/Chick β-actin (CAG)

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135766

    Purpose

    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    

    Use

    Zebrafish

    Promoter

    UAS

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. 18065-M02-412

    Plasmid

    #225665

    Purpose

    Lentiviral expression of FLAG-tagged fluorescent proteins for immunohistochemical detection in FFPE tissue

    Depositor

    Dominic Esposito

    Article

    Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

    Inserts

    dsmCherry
    delCD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Mutation

    C-terminally truncated (aa 1-333 only)

    Promoter

    SORE6-mCMVp and WPRE-SV40p

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGMC00027

    Plasmid

    #199279

    Purpose

    SpCas9 construct to knockout murine Cd274

    Depositor

    Raj Chari

    Article

    Ruf et al Cell. 2023 Aug 17;186(17):3686-3705.e32.

    Insert

    Cd274 guide RNA (Cd274 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. BL21(DE3)-R3-pRARE2

    Bacterial Strain

    #26242

    Purpose

    Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Bacterial Resistance

    Chloramphenicol

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pWZL Sox2

    Plasmid

    #26351

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pBABE FoxE1

    Plasmid

    #26350

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    FOXE1 (FOXE1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only