We narrowed to 5,303 results for: pcDNA3.1+

1. ER2-LSSmGFP

   Plasmid

   #186538

   Purpose

   Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LacI INCENP

   Plasmid

   #45236

   Purpose

   Target INCENP to lac operator sequence

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   LacI, INCENP-Δcen, mCherry (lacI )
   

   Use

   Tags

   LacI (lac repressor protein) and mCherry

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   t7

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-ALFA-tag-Gal4-VP16

   Plasmid

   #199465

   Purpose

   Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

   Insert

   Gal4-ALFA-tag-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEQ1007

   Plasmid

   #83938

   Purpose

   express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

   Depositor

   Adam Geballe

   Article

   Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

   Insert

   IRS1
   

   Use

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   R833L in IRS1 (please see depositor comment)

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcPylRS(Y384F Y306A)

   Plasmid

   #196486

   Purpose

   To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TR therapy reporter

   Plasmid

   #179083

   Purpose

   Fluorescent reporter for alternative splicing

   Depositor

   Thurman Wheeler

   Article

   Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

   Inserts

   DsRed1
   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA_RFP-SZ2

   Plasmid

   #124936

   Purpose

   Expression of RFP-SZ2

   Depositor

   Matthew Good, Benjamin Schuster, Daniel Hammer

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RFP-SYNZIP2
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   SZ2 domain

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmiRFP670nano3-NbGFP

   Plasmid

   #184686

   Purpose

   Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-NbGFP
   

   Use

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZ143-pcDNA3-BvCas12b

   Plasmid

   #122445

   Purpose

   Expresses BvCas12b in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   CMV-NLS-BvCas12b-NLS-3xHA
   

   Use

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rtn3c-mEmerald

    Plasmid

    #186963

    Purpose

    Express Rtn3c with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    RTN3C (Rtn3 Mouse)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-ClopHensor-H148G-V224L

    Plasmid

    #46366

    Depositor

    Daniele Arosio

    Article

    Mukhtarov et al Front Mol Neurosci. 2013 Apr 18;6:9. doi

    Insert

    ClopHensor-H148G-V224L
    

    Use

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

12. TNXXL-CTS20

    Plasmid

    #48342

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    CTS20
    

    Use

    Tags

    TN-XXL

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOVC1_X_ScPH1-S

    Plasmid

    #131193

    Purpose

    Expresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    ScPH1-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. tCRMod-GGSGGS7

    Plasmid

    #111761

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS7-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3-MARS-p85(iSH2)

    Plasmid

    #205236

    Purpose

    Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

    Depositor

    Jeremy Baskin

    Inserts

    PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
    p85(iSH2) (Pik3r1 Mouse)
    

    Use

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. tCRMod-GGSGGS5

    Plasmid

    #111760

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS5-mRuby2 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. [Bla][IRES][GFP]

    Plasmid

    #97193

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Integrin-αv Ectodomain M400C

    Plasmid

    #159642

    Purpose

    Contains integrin alpha v residues 1–960 with the M400C mutation

    Depositor

    Timothy Springer

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

    Insert

    Integrin alpha v ectodomain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M400C

    Promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mGarnet2-RITA

    Plasmid

    #104314

    Purpose

    codes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

    Depositor

    Ulrich Nienhaus

    Article

    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

    Insert

    mGarnet2-RITA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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