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Activation of the thrombopoietin receptor by mutant calreticulin in CALR-mutant myeloproliferative neoplasms.
Araki M, Yang Y, Masubuchi N, Hironaka Y, Takei H, Morishita S, Mizukami Y, Kan S, Shirane S, Edahiro Y, Sunami Y, Ohsaka A, Komatsu N
Blood. 2016 Jan 27. pii: blood-2015-09-671172. PubMed Article

Plasmids from Article

ID Plasmid Purpose
204523pMSCV-IRES-GFP/CALR del52-FLAGRetroviral expression of human CALR del52-FLAG
204524pMSCV-IRES-GFP/CALR ins5-FLAGRetroviral expression of human CALR ins5-FLAG
204525pMSCV-IRES-GFP/CALR wt-FLAGRetroviral expression of human CALR wt-FLAG
204526pLKO.3R/MPL-shRNA1Lentiviral expression of MPL-shRNA1; gene knock down of human MPL
204527pLKO.3R/MPL-shRNA2Lentiviral expression of MPL-shRNA2; gene knock down of human MPL
204528pLKO.3R/MPL-shRNA3Lentiviral expression of MPL-shRNA3; gene knock down of human MPL
204529pLKO.3R/MPL-shRNA4Lentiviral expression of MPL-shRNA4; gene knock down of human MPL
204530pcDNA3.1/MPL-HAMammalian expression of human MPL-HA
204531pcDNA3.1/MPL-V5Mammalian expression of human MPL-V5
204532pcDNA3.1/CALR wt-FLAGMammalian expression of human CALR wt-FLAG
204533pcDNA3.1/CALR ins5 (del1-17)-FLAGMammalian expression of human CALR ins5 (del1-17)-FLAG
204534pcDNA3.1/CALR ins5 (del1-197)-FLAGMammalian expression of human CALR ins5 (del1-197)-FLAG
204535pcDNA3.1/CALR ins5 (del1-308)-FLAGMammalian expression of human CALR ins5 (del1-308)-FLAG
204536pcDNA3.1/CALR ins5 (del367-430)-FLAGMammalian expression of human CALR ins5 (del367-430)-FLAG
204537pcDNA3.1/CALR ins5 (del309-430)-FLAGMammalian expression of human CALR ins5 (del309-430)-FLAG
204538pcDNA3.1/CALR ins5 (del198-430)-FLAGMammalian expression of human CALR ins5 (del198-430)-FLAG
204539pcDNA3.1/CALR ins5 (del18-197)-FLAGMammalian expression of human CALR ins5 (del18-197)-FLAG
204540pcDNA3.1/CALR ins5 (del198-308)-FLAGMammalian expression of human CALR ins5 (del198-308)-FLAG

Antibodies from Article