We narrowed to 5,339 results for: pcdna3.1

1. LacI INCENP

   Plasmid

   #45236

   Purpose

   Target INCENP to lac operator sequence

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   LacI, INCENP-Δcen, mCherry (lacI )
   

   Tags

   LacI (lac repressor protein) and mCherry

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   t7

   Available Since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TR therapy reporter

   Plasmid

   #179083

   Purpose

   Fluorescent reporter for alternative splicing

   Depositor

   Thurman Wheeler

   Article

   Hu et al Nat Commun. 2018 Dec 7;9(1):5227. doi: 1

   Inserts

   DsRed1
   EGFP
   

   Expression

   Mammalian

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Fra-1

   Plasmid

   #187906

   Purpose

   Expresses Fra-1 in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

   Insert

   Fra1 (Fosl1 Rat)
   

   Expression

   Mammalian

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVH233-Tier1-PmPGK1-eYFP

   Plasmid

   #169522

   Purpose

   Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven enhanced yellow fluorescent protein
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA_RFP-SZ2

   Plasmid

   #124936

   Purpose

   Expression of RFP-SZ2

   Depositor

   Benjamin Schuster, Daniel Hammer, Matthew Good

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RFP-SYNZIP2
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   SZ2 domain

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZ143-pcDNA3-BvCas12b

   Plasmid

   #122445

   Purpose

   Expresses BvCas12b in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   CMV-NLS-BvCas12b-NLS-3xHA
   

   Tags

   3XHA

   Expression

   Mammalian

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEQ1007

   Plasmid

   #83938

   Purpose

   express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

   Depositor

   Adam Geballe

   Article

   Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

   Insert

   IRS1
   

   Tags

   6xHIS

   Expression

   Mammalian

   Mutation

   R833L in IRS1 (please see depositor comment)

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmiRFP670nano3-NbGFP

   Plasmid

   #184686

   Purpose

   Nanobody to GFP labelled with near-infrared fluorescent protein miRFP670nano3

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

   Insert

   miRFP670nano3-NbGFP
   

   Tags

   miRFP670nano3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcPylRS(Y384F Y306A)

   Plasmid

   #196486

   Purpose

   To express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.

   Depositor

   Kensaku Sakamoto

   Article

   Hino et al Methods Mol Biol. 2012;794:215-28. doi:

   Insert

   pyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-ClopHensor-H148G-V224L

    Plasmid

    #46366

    Depositor

    Daniele Arosio

    Article

    Mukhtarov et al Front Mol Neurosci. 2013 Apr 18;6:9. doi

    Insert

    ClopHensor-H148G-V224L
    

    Tags

    Strep-II

    Expression

    Mammalian

    Mutation

    E2GFP-H148G-V224L is EGFP-T203Y-H148G-V224L

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TNXXL-CTS20

    Plasmid

    #48342

    Purpose

    FRET reporter for Ca2+ inside primary cilia

    Depositor

    Takanari Inoue

    Article

    Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

    Insert

    CTS20
    

    Tags

    TN-XXL

    Expression

    Mammalian

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

13. Rtn3c-mEmerald

    Plasmid

    #186963

    Purpose

    Express Rtn3c with C-terminus mEmerald

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    RTN3C (Rtn3 Mouse)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. tCRMod-GGSGGS7

    Plasmid

    #111761

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS7-mRuby2 fusion
    

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. tCRMod-GGSGGS5

    Plasmid

    #111760

    Purpose

    Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-GGSGGS5-mRuby2 fusion
    

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOVC1_X_ScPH1-S

    Plasmid

    #131193

    Purpose

    Expresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    ScPH1-S
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. [Bla][IRES][GFP]

    Plasmid

    #97193

    Purpose

    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Inserts

    beta-Lactamase
    GFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. optGluCl beta Y182F ECFP

    Plasmid

    #15105

    Depositor

    Henry Lester

    Article

    Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

    Insert

    GluCl beta (glc-2 Nematode)
    

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA, Y182F

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3-MARS-p85(iSH2)

    Plasmid

    #205236

    Purpose

    Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

    Depositor

    Jeremy Baskin

    Inserts

    PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
    p85(iSH2) (Pik3r1 Mouse)
    

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Integrin-αv Ectodomain M400C

    Plasmid

    #159642

    Purpose

    Contains integrin alpha v residues 1–960 with the M400C mutation

    Depositor

    Timothy Springer

    Article

    Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

    Insert

    Integrin alpha v ectodomain
    

    Expression

    Mammalian

    Mutation

    M400C

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge