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pEB0_13_PGK1p-QF-ACT1t

Plasmid

#236216

Purpose

Plasmid encoding PGK1p-QF2-ACT1t as a combined Type 234 part to be used in the Dueber Yeast MoClo Toolkit.

Depositor

Yvonne Nygård

Article

Blick et al Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf0

Insert

QF, ACT1 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PGK1p

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pRS426-SNR52-crRNA

Plasmid

#251984

Purpose

Expression of crRNA (version 3) for editing, used together with tracrRNA Plasmid #251985. This plasmid is also called crRNA in the paper.

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

crRNA

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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HRPEadh-pAG303

Plasmid

#249735

Purpose

Derived from pAG303GPD-ccdb (#14134); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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HRPEadh-pAG305

Plasmid

#249734

Purpose

Derived from pAG305GPD-ccdb (#14138); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAG304-ccdbNLUC

Plasmid

#249736

Purpose

Derived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiae

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-AtAFB2

Plasmid

#247611

Purpose

Expression of AtAFB2

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

AtAFB2

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNH605-Padh-OsTIR1

Plasmid

#247609

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRS405-Padh-AtAFB2

Plasmid

#247600

Purpose

Expression of AtAFB2

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

AtAFB2

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRO288

Plasmid

#233827

Purpose

MoClo-YTK; type4A; Activation domains; VP48

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

VP48

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO129

Plasmid

#233805

Purpose

MoClo-YTK; type3; rtTA systems; rtTA3

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

rtTA3

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO232

Plasmid

#233808

Purpose

MoClo-YTK; type2A; ABA systems; phlfO7

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

phlfO7

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO175

Plasmid

#233810

Purpose

MoClo-YTK; type3B; ABA systems; ABI

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

ABI

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO177

Plasmid

#233811

Purpose

MoClo-YTK; type3B; ABA systems; PYL1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

PYL1

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO286

Plasmid

#233812

Purpose

MoClo-YTK; type2A; Auxin systems; amtRO7

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

amtRO7

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN022

Plasmid

#233814

Purpose

MoClo-YTK; type3B; Auxin systems; AIDΔ34

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

AIDΔ34

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN079

Plasmid

#233819

Purpose

MoClo-YTK; type3B; NS3 systems; NS3a

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3a

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN043

Plasmid

#233821

Purpose

MoClo-YTK; type3B; NS3 systems; DNCR2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

DNCR2

Expression

Yeast

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-L1374

Plasmid

#226278

Purpose

Plasmid expressing the SSD1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII41K-TDH3pr

Plasmid

#194528

Purpose

Low copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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