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Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
Hayashi A, Tanaka K
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
121144pAH233Expression of gRNA with BbsI placeholder for target cloning
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121145pAH235Cas9 expression controlled by the nmt41 promoter
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121146pAH237Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast
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121147pAH243Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast
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