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pcDNA3 WT MYOC eGLuc2 FLAG

Plasmid

#192820

Purpose

Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

Depositor

John Hulleman

Article

Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

Insert

myocilin (MYOC Human)

Tags

FLAG and enhanced Gaussia luciferase (M43I/M110I)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2

Plasmid

#183744

Purpose

Expresses Ki67 (shuffled Nterm)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (shuffled Nterm) (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

CD domain with shuffled 4 fragments fused to the …

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-Control (pSLIK3)

Plasmid

#113858

Purpose

An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

E2-Crimson

Use

Lentiviral

Tags

P2A/Crimson

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAW2-Smed-Slit-1

Plasmid

#182264

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Insert

Smed-Slit-1

Use

Gibson cloning vector for synthesis of single and…

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL1994

Plasmid

#139898

Purpose

Expression plasmid for LetB PLL2 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta229-239, D228G, L240G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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U6-U57C

Plasmid

#169928

Purpose

WT U6 with a U57C mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57C

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTLR-donor

Plasmid

#118999

Purpose

Repair template for the TLR reporter

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTF-R

Plasmid

#139669

Purpose

Expression cassette for R recombinase in T-DNA vector

Depositor

James Birchler

Article

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

R recombinase

Expression

Plant

Promoter

Maize Ubiquitin

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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Tmem98-mTq2

Plasmid

#124504

Purpose

Expresses mTurquoise2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Tags

mTurquoise2

Expression

Mammalian

Mutation

stop codon was removed to allow fusion with sGFP2…

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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Tmem98-mCherry

Plasmid

#124505

Purpose

Expresses mCherry-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

stop codon was removed to allow fusion with sGFP2…

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Frat1

Plasmid

#124499

Purpose

Expresses myc-tagged mouse FRAT1 in mammalian cells (N-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Frat1 (Frat1 Mouse)

Tags

myc tag

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Frat2

Plasmid

#124500

Purpose

Expresses myc-tagged mouse FRAT2 in mammalian cells (N-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Frat2 (Frat2 Mouse)

Tags

myc tag

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET29b-pld

Plasmid

#128795

Purpose

expresses E. coli PLD in bacteria

Depositor

Emily Balskus

Article

Chittim et al Nat Microbiol. 2019 Jan;4(1):155-163. do

Insert

Phospholipase D

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJL042

Plasmid

#122865

Purpose

Binary expression vector containing 35S:MS2-KYP:tRBCS followed by GreenGate A-G sequences for insertion of additional modules

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

35S:MS2-KYP:tRBCS

Use

Golden gate compatible plant transformation vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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Ubiquitin (1-76; L71Y)

Plasmid

#110756

Purpose

Bacterial expression of human Ubiquitin (1-76; L71Y)

Depositor

David Komander

Article

Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

Insert

UBB (UBB Human)

Expression

Bacterial

Mutation

L71Y

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dSesn (1187)

Plasmid

#66919

Purpose

bacterial expression of dSesn

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn (Sesn Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN51 (R40A)

Plasmid

#80109

Purpose

Late1-O3-33-myc-PH(R40A)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (R40A)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-25 (1wa3)

Plasmid

#80124

Purpose

I3-01p-myc-Late1-PH (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-25 (1wa3)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-20

Plasmid

#79935

Purpose

Late2-4GS-I3-01-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-20

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01

Plasmid

#79916

Purpose

Myr1-12GS-I3-01-myc-Late1

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only