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We narrowed to 773 results for: Dos

Showing: 1 - 20 of 773 results

  1. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a... vitro transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should...

  2. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a...vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other plasmids that should...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...Systems, Harvard University.    Which titer do people usually report? Addgene and other viral vector...values to determine accuracy and reliability. When doing this, we make a judgement call on whether the two...improvement over qPCR (Taylor et al., 2017). What do Addgene AAV titers mean for your experiments? Because...reordering the same virus feel free to email us and we can do our best to send you a particular lot (if we still...Figure 2). Because physical titers are used for dosing purposes in preclinical studies, it is important...each lot of AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but if you’...

  5. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...repression experiments use targets within promoters. Do you have gRNAs you'd like to add to the Addgene collection...ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...immunoprecipitations of your favorite gene even if you do not have an antibody against it. Another common...incorporated into the host DNA. For mammalian cells, you can do this through viral vector delivery. Visit our... Backbones AAV High transduction efficiency, but do NOT integrate into the host genome Addgene has an...Addgene Adenoviral High transduction efficiency, but do NOT integrate into host genome pAdTrack-CMV... Return to top Content last reviewed 28 May 2025. Do you have suggestions for other plasmids that should...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created by directed...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

  8. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a...Ingestion by C. elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids that should...

  9. AAV for Neuronal Tracing

    Type
    Collection
    ... RABV. These projection neurons do not express G-protein, and thus do not give rise to G-protein coated...

  10. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a...Cas9 plasmid O'Connor-Giles , Harrison , Wildonger Do you have suggestions for other plasmids that should...

  11. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...But how do you know if a masters will lead to a career you will like? Here’s two things to do to answer...decide to earn a PhD, but if I had to do grad school over, I would just do the master’s! If you'd like more...blog Learn about career planning resources What Do I Do Now? Academic v. Non-Academic Career Decisions ...wasn’t sure a master’s was a good decision. So how do you know if you should earn a master? Here are a ...job postings on a job search website like Indeed. Do the job descriptions sound interesting? Is the work... to see how people describe their actual work. To do this, try looking at LinkedIn profiles of people ...interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound interesting? Is their career path...

  12. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...consider. What do academic and non-academic careers in science have in common? You can do quality science... to work/life scheduling Diverse opportunities to do science related jobs that require no bench work Better...available ($)—can outsource a task someone else who can do better, no grant writing Job changes can happen regularly...

  13. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...things I’ll be doing through the course of any given week. Knowing this, I hand write to-do lists at the...Probably the easiest way to do this is to leave your phone in your bag on “Do Not Disturb” during the times...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...Time Mangement for Scientists. Back when I used to do mammalian cell culture, I would often respond to ...complete the task When picking which tasks to actually do, you should err on the side of things that have the...likely energize you. For example, let's say you’re doing a multi-day experiment in the lab. You have two ...but you’ll have to spend the entirety of each day doing the experiment. The second way, it will take you...

  14. CRISPR Plasmids - Yeast

    Type
    Collection
    ...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a...HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do you have suggestions for other plasmids that should...

  15. Viral Vector Guides and Plasmids

    Type
    Collection
    ...research typically do not have the ability to propagate in the same way that wild-type viruses do. For general...

  16. Scientist Networking: What is an Informational Interview?

    Type
    Blog Post
    ...the best things you can do during your training is meet interesting people doing interesting things. I ...you want to pursue someone doing a job you wish you knew more about – how do you make a connection? An ...position at their company, do NOT call this an Informational Interview. If you do this, the connection may...graduate degree in science. I find it ironic that we do all of our training as scientists (5-12 years worth...about learning new things from other scientists doing interesting work. Consider this to be like any other...manager. They might say yes and they might say no but do not confuse networking for a job connection with ...not possible consider a phone or video call. If you do decide to use Skype, make sure to follow the rules...

  17. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...you may need to find a way to do, manage and lead all in one day.  How do you convince others (in particular...all career skills leaderhsip skills when done well? Do you just have to know it when you see it? “The Three...any stage in our careers we may be in a cycle of doing, managing or leading. All three roles are important... or finishing any project. Doers are the ones who do the work Doers focus on understanding what is expected....  Managers make sure the work gets done Managers do this by removing obstacles that stand in the way ...with other teams. While they sometimes dig in and do some of the work, they must be more focused on delegating...three roles allows you to identify what you need to do to be successful in a specific role or cycle. For...

  18. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...fairs is a great start, but you need to do more than just show up. Do your homework and know who is going ...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...end, one of the most important things that you can do now is diversify. Read on for an overview of some...Further, even for those students and researchers who do take the initiative to develop themselves, resources...difficult to identify. So what specifically should you do? What follows are just a few suggestions based on...Conferences One of the single most important things you can do to plan for your career is to expose yourself to ...to other working professionals, and one way to do this is to attend conferences. Depending upon your discipline...

  19. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...overwhelmed with your to-do list and your team doesn’t seem to have enough to do…you are not delegating ...and experience? Do you think you are giving this responsibility to someone who can do the job. It is good... responsibilities. The manager is not expected to DO all the work – she is expected to make sure the work...imperative that you resist the temptation to “just do it myself” or micromanage –you don’t have time for...that there are always things that the manager must do. You can’t delegate the giving of feedback or performance... good to delegate so the person doing the task will have to learn and stretch a little, but be careful... up for failure by delegating is a bad idea. How do I make sure it goes well? As with most managerial...

  20. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...experimental steps are worth doing, and doing well, so make sure you’re taking time to do them carefully and thoroughly... significantly impacts your experiments. What do you do when you’re in the lab and you're sweating the...for doing things accurately. Thankfully, you should already have a checklist for everything you do in ...make a mistake, instead of silently vowing never to do that again (I’ve been there!), think of ways you ... can be incredibly tedious, but it also works, so do it anyway.  You may find that after running a protocol... to focus, a large set of headphones can act as a Do Not Disturb sign, even if the headphones aren’t actually...
Showing: 1 - 20 of 773 results