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We narrowed to 12 results for: Dos

Showing: 1 - 12 of 12 results

  1. Chemogenetics Guide

    Type
    Guide
    ...coupled receptors? Read the GPCRs: How Do They Work and How Do We Study Them? blog! Studies of GPCRs led...are engineered to respond to small molecules that do not affect endogenous signaling, therefore allowing...DCZ seem to have minimal off-target activity, and do not seem to have the same back-metabolism issues ... consider: Chemogenetic activation or inhibition. Do you want to turn ON or turn OFF neurons in your experiment...high potency, which is potentially important for dosing in animal studies, made them attractive targets...

  2. Adenovirus Guide

    Type
    Guide
    ...double-stranded DNA (dsDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle...cells for rAdV vector production, as HEK293 cells do not contain E4. Download the Bert Vogelstein’s lab...infection, wild-type adenovirus as well as rAdV vectors do not integrate into the host genome. Instead, they...cell line. Recombinant adenoviral vectors typically do not integrate in the host genome and instead are ...for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV...

  3. Cloning

    Type
    Guide
    ...plasmids for replication and protein expression. So how do scientists recombine DNA? There are many methods ...so dissimilar from restriction enzyme cloning). To do this your DNA fragment must first be amplified with...move it into a series of plasmids that allow you to do many different molecular biology techniques (such...

  4. Guide to Using Pooled Libraries

    Type
    Guide
    ...: Apply library Apply selection Most cells die or do not pass selection (in the case of a reporter) Sort... screen, the goal is to identify those cells that do not survive the selection mechanism. You’ll infect...

  5. Antibody Guide

    Type
    Guide
    ...comes from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will...direct or indirect detection method? If indirect, do you already have secondary antibodies available in...have identical features and epitopes, to ensure you do not validate the same antibody multiple times. You...antibodies will also have a source species. What conjugate do you want to use? Most secondary antibodies will be...buy small aliquots of many different antibodies and do batch validation up front rather than risk delaying...

  6. Molecular Biology Reference

    Type
    Guide
    ...function and the correct gene of interest. So how do scientists sequence DNA? Sanger Sequencing In 1975...fluorescently tagged nucleotide. Because these special bases do not have a binding site for adding the next nucleotide... can be converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does...is important to note that NGS platforms in general do not require a specific primer for your DNA of interest...

  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...single-stranded DNA (ssDNA) genome. Non-enveloped viruses do not have a lipid bilayer surrounding the viral particle.... AAVs are therefore non-pathogenic and generally do not trigger a strong immune response. These features... genome for viral vector production, rAAV vectors do not carry the genes to express Rep78 and Rep 68 proteins...vectors present low oncogenic potential because they do not integrate in the human chromosome, which reduces...

  8. Plan Your Experiment

    Type
    Guide
    ...model your specific disease or process of interest. Do you want to: Generate complete and permanent loss...percentage of DSBs are still repaired by NHEJ. How do you determine that your desired edit has occurred...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-retroviral vectors do not have defined “generations” of plasmids, as lentiviral vectors do. For more about...

  10. CRISPR Guide

    Type
    Guide
    ...to G (or T to C) change. Adenosine DNA deaminases do not exist in nature, but have been created by directed...have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR libraries from Addgene...alterations, such as small mutations or inversions, than do large deletions generated by Cas9 systems. Cas3 must..., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...

  11. Optogenetics Guide

    Type
    Guide
    ...excitation or optogenetic inhibition. First things first: do you want to turn ON or turn OFF neurons in your experiment...Examples: iChloC, SwiChRca, Phobos, Aurora Browse Channelrhodospin plasmids . Halorhodopsins Halorhodopsins are...

  12. Lentiviral Vector Guide

    Type
    Guide
    ...system in total. In addition, third-generation systems do not require the HIV protein Tat to activate the promoter...
Showing: 1 - 12 of 12 results