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Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

Description of Polymerase Chain Reaction with protocol, tips and FAQ

Protocol - How to Ligate Plasmid DNA

Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.

Protocols for plasmid cloning by PCR.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more.

These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely.

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Protocol for Inoculating a Bacterial Culture

These guidelines summarize the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

Learn how to streak bacteria on an LB agar plate to obtain single colonies.

Best practices for performing sequence analysis of a plasmid

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Use this protocol to prepare LB agar plates with antibiotic in your lab.

Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker.