We narrowed to 5,344 results for: biorxiv

1. HA-MBP-TEVpCS-ADGRE3 CTF-FLAG

   Plasmid

   #217691

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE3 (ADGRE3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-338

   Promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-MBP-TEVpCS-ADGRE2 CTF-FLAG

   Plasmid

   #217690

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE2 (ADGRE2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-517

   Promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG

   Plasmid

   #217723

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE2 (ADGRE2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-523

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

   Plasmid

   #222989

   Purpose

   Bacterial expression of iGeoCas9(G) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   CL7_2xNLS_iGeoCas9(G)_2xNLS_His
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mc2 mCherry EF1a

   Plasmid

   #206225

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Brett Stringer, Simon Conn

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GALT-APEX2

   Plasmid

   #215554

   Purpose

   Stable, inducible expression in Golgi

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   APEX2-NES-GalT-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LBR-FKBP-GFP

   Plasmid

   #222413

   Purpose

   To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

   Depositor

   Stephen Royle

   Article

   Downie et al bioRxiv 2024.05.31.596797

   Insert

   LBR (LBR Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

   Plasmid

   #202025

   Purpose

   Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpB S9G-R31L-R85L

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

   Plasmid

   #216385

   Purpose

   Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Norrin (NDP Human)
   

   Use

   Baculovirus

   Tags

   GP64 signal sequence-MBP-3C and Rho1D4

   Expression

   Insect

   Mutation

   C93A/C95A/C131A

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pICH-nuc-roGFP2-GPXC71S

    Plasmid

    #191760

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    GPX has no peroxidatic Cysteine 71, which is repl…

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE

    Plasmid

    #218760

    Purpose

    Provides AND intersectional (Cre+Flp) expression of ArchT-eGFP

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    ArchT
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CMV-dSa-VPR mini.-syn pA

    Plasmid

    #99689

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mc2 neo EF1a

    Plasmid

    #206220

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-MBP-TEVpCS-ADGRF4 CTF-FLAG

    Plasmid

    #217697

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF4 (ADGRF4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-384

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

    Plasmid

    #217689

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE1 (EMR1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-584

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFR-mTq2-antiFRET-P2A-jRCaMP1b

    Plasmid

    #191468

    Purpose

    Compare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent protein

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    jRCaMP1b
    

    Use

    Tags

    His-mTurquoise2-antiFRETlinker-P2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mc2 blast EF1a

    Plasmid

    #206223

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mc2 mCherry

    Plasmid

    #206219

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TL021_lenti_CMV:SEP-GluA1

    Plasmid

    #192515

    Purpose

    Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

    Depositor

    Adam Cohen

    Article

    Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

    Insert

    SEP-GluA1
    

    Use

    Lentiviral

    Tags

    super ecliptic phluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mc2 neo

    Plasmid

    #206214

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge