We narrowed to 4,097 results for: biorxiv

1. PGK Promoter (MTK 2) (pAN2822)

   Plasmid

   #194224

   Purpose

   Mammalian Toolkit part 2 encoding the PGK promoter

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   PGK Promoter
   

   Use

   Synthetic Biology

   Promoter

   PGK

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF1a Promoter (MTK 2) (pAN2824)

   Plasmid

   #194225

   Purpose

   Mammalian Toolkit part 2 containing the EF1a promoter

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   EF1a Promoter
   

   Use

   Synthetic Biology

   Promoter

   EF1a

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

   Plasmid

   #223146

   Purpose

   Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   NIPP1 (PPP1R8 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 143-224

   Promoter

   EF1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBK1311-AAV-EFSNC-dSaCas9-NIPP1(143-224)

   Plasmid

   #223156

   Purpose

   Expression of truncated NIPP1 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   NIPP1 (PPP1R8 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 143-224

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330_NPM2_cterm1

   Plasmid

   #222911

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 1 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_NPM2_cterm4

   Plasmid

   #222914

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 4 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_NPM2_cterm3

   Plasmid

   #222913

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 3 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHDR2_TFAP2C-T2A-mGreenLantern-Hygro

   Plasmid

   #222915

   Purpose

   Homology directed repair template for knocking in mGreenLantern reporter to TFAP2C.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mGreenLantern
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330_NPM2_cterm2

   Plasmid

   #222912

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 2 (NPM2 Human, Synthetic)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

    Plasmid

    #220494

    Purpose

    To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting LIG4 exon 3 (LIG4 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

    Plasmid

    #195018

    Purpose

    An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

    Depositor

    Christopher Richie

    Article

    Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

    Inserts

    CCGTATAGGCGGCTGCCCAA
    EGFP-KASH
    TTCCGTGGTGGGCAACGTAG
    

    Use

    AAV

    Tags

    KASH domain of Nesprin2

    Promoter

    EF1a, hU6, and mU6

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1_oROS-HT_LF(C199S)

    Plasmid

    #216412

    Purpose

    Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT_LF(C199S)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC1-lifeact-oROS-HT

    Plasmid

    #216420

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    lifeact-oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pToxEXPr6

    Plasmid

    #218290

    Purpose

    Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJQR3

    Plasmid

    #218289

    Purpose

    Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pITEdv1

    Plasmid

    #218283

    Purpose

    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pITEu2

    Plasmid

    #218282

    Purpose

    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJE13HD7

    Plasmid

    #218281

    Purpose

    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

    Depositor

    Claudia Vickers

    Article

    Evans et al bioRxiv 2024.01.30.578080

    Insert

    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mouse b6 full length

    Plasmid

    #217816

    Purpose

    Full length mouse integrin b6 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGB6 (Itgb6 Mouse)
    

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. eGFP-SUN1ΔN

    Plasmid

    #213508

    Purpose

    Expresses human eGFP-SUNΔN in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    eGFP-SUNΔN
    

    Expression

    Mammalian

    Mutation

    SUN1ΔN has the first 138 amino acid (lamina domai…

    Promoter

    CMV

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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