We narrowed to 5,092 results for: codon optimized

1. pX330-Flag-HeFSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126759

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126753

   Purpose

   Expression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-Flag-eSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126754

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-SpCas9-HF1 (without sgRNA; with silent mutations)

   Plasmid

   #126755

   Purpose

   Expression plasmid for human codon-optimized increased fidelity SpCas9-HF1 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126756

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HypaSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PV259

   Plasmid

   #132340

   Purpose

   CasD plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   CasD-AT-CBF1 (CBF1 CasD (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
   

   Use

   Tags

   AT-CBF1

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PV503

   Plasmid

   #132342

   Purpose

   CasE plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   CasE-AT-CBF1 (CBF1 Mustard Weed, CasE (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana))
   

   Use

   Tags

   AT-CBF1

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. PV504

   Plasmid

   #132343

   Purpose

   CasA plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   CasA-AT-CBF1 (CBF1 CasA (Streptococcus thermophilus); AT-CBF1 (Arabidopsis thaliana), Mustard Weed)
   

   Use

   Tags

   AT-CBF1

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCEP4-191084-QES.i06.c07

   Plasmid

   #123239

   Purpose

   Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; QES mutant with enhanced binding to PG16

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (191084 B7-19) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Mutations P124D, …

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-4T-3-cytXlATL2

    Plasmid

    #124063

    Purpose

    Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXIATL2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCEP4-Q842-QES.i04.c05

    Plasmid

    #123233

    Purpose

    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations P124D, …

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCEP4-Q769-QES.i03.c04

    Plasmid

    #123229

    Purpose

    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q769.d22) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations A200E, …

    Promoter

    CMV

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCEP4-H031-QES.i07.c09

    Plasmid

    #123271

    Purpose

    Mammalian expression plasmid for Env from the H031 HIV-1 isolate; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (H031) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations S49D, F…

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCEP4-SHIV327C-HuA10-QES.i09.c07

    Plasmid

    #123269

    Purpose

    Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    SHIV327C-Hu A10 Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; Mutations V208M, …

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-Q842gp160-QES.04

    Plasmid

    #100933

    Purpose

    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; mutations P124D;F…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-Q842gp160-QES.i04.I181L

    Plasmid

    #111837

    Purpose

    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q842.d12) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; mutations P124D;I…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4-Q769gp160-QES.i03.V255M

    Plasmid

    #111838

    Purpose

    Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q769.d22) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; mutations A200E;F…

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMXs_FLAG-Sfxn2 (CG6812)

    Plasmid

    #110643

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG6812 (CG6812 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hSRAP.HMG6.wt.Dlnk.a13-236

    Plasmid

    #49814

    Purpose

    expression vector for MBP-SRAP(13-236) fusion

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator protein (SRA1 Human)
    

    Use

    Tags

    E. coli Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    wildtype codon-optimized for E. coli expression

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HsCLYBL(aa23-340) D320N-His

    Plasmid

    #111289

    Purpose

    bacterial expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    human CLYBL ( aa23-340) D320N codon optimized for bacterial expression (CLYBL Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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