We narrowed to 3,680 results for: golden gate

1. pAGT10446

   Plasmid

   #192353

   Purpose

   L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   TTCG_Stop-t35S_CGCT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGT5439

   Plasmid

   #192351

   Purpose

   L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   TTCG_Stop-tOCS_CGCT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAGT9403

   Plasmid

   #192352

   Purpose

   L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   TTCG_Stop-tNOS_CGCT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. JME4390

   Plasmid

   #129656

   Purpose

   LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. JME4393

   Plasmid

   #129657

   Purpose

   LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSEED-Moontag:HA

   Plasmid

   #219648

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   MoonTag:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEED-EYFP:HA

   Plasmid

   #219656

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   EYFP:HA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JME5000

   Plasmid

   #129661

   Purpose

   EYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEED-LoxP-sfGFP:ALFA

   Plasmid

   #219660

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP:ALFA SEED Cassette (LoxP)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Level 1 P2 OsUbiP:Cas9:NosT

    Plasmid

    #165424

    Purpose

    GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Os Ubiquitin promoter :: Cas9 :: NosTerminator

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEED-mScarlet3:ALFA

    Plasmid

    #219641

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet:ALFA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZmSWEET13a:ZmSWEET13a:GUS

    Plasmid

    #159535

    Purpose

    expresses ß--glucuronidase in cells expressing ZmSWEET13a

    Depositor

    Wolf Frommer

    Article

    Bezrutczyk et al Plant Cell. 2021 May 5;33(3):531-547. do

    Insert

    ZmSWEET13a
    

    Use

    Tags

    GUS

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    native SWEET13a promoter

    Available since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

    Plasmid

    #192350

    Purpose

    L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    AATG_dttLbCas12a-i_TTCG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R; E925Q

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJT050 lenti pEF-rTetR-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid

    #161925

    Purpose

    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGGG_Sr1644-1Sh

    Plasmid

    #164087

    Purpose

    Expresses pGGG_Sr1644-1Sh in plants

    Depositor

    Brande Wulff

    Article

    Yu et al Nat Commun. 2022 Mar 25;13(1):1607. doi:

    Insert

    pGGG_Sr-1644-1Sh
    

    Use

    Tags

    none

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Native promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid

    #161926

    Purpose

    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    rTetR(SE-G72P)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTK Cas9-2A-Citrine

    Plasmid

    #92393

    Purpose

    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    thymidine kinase promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGG L2 TaStb15

    Plasmid

    #226630

    Purpose

    Wheat transformation vector used to express wheat Septoria tritici blotch (Stb) resistance gene 15

    Depositor

    Brande Wulff

    Article

    Hafeez et al Nat Plants. 2025 Mar;11(3):410-420. doi:

    Insert

    Triticum aestivum (wheat) Septoria tritici blotch 15 (TaStb15) disease resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Native TaStb15

    Available since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LbPlant toolbox II

    Plasmid Kit

    #1000000263

    Purpose

    Use the LbPlant Toolbox kits for the rapid construction of expression vectors for Lactiplantibacillus plantarum using Golden Gate cloning.

    Depositor

    Georg Fritz

    Available since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

20. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit

    #1000000030

    Purpose

    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

    Depositor

    Takashi Yamamoto

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only