We narrowed to 18,480 results for: gateway

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pLenti-BLAST-CMV-LiCre-WPRE (JDW 1495)

Plasmid

#242594

Purpose

Lentiviral vector with a CMV promoter driving a light inducible cre (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-EFS-mODC-rtTA-IRES-NEO (JDW 931)

Plasmid

#242578

Purpose

PiggyBac transposon flanked, Tet-on, gateway destination vector.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DV-IRES-myr-mKate (JDW 494)

Plasmid

#242575

Purpose

Gateway destination vector with a CAGGS promoter in the backbone as well as an IRES-myr-mKate to label cell membranes.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attR1-CmR-ccdB-attR2

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

Plasmid

#242587

Purpose

A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nlsKateV5, MbBPF-FLAG, FRTNeoFRT

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-hs-miR-126 (JDW 1415)

Plasmid

#242581

Purpose

An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with an intron containing hs-miR-126 (MIR126 Human)

Expression

Mammalian

Promoter

human EF1a

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

Plasmid

#229827

Purpose

A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xnls-TagBFP2-MCS-FLAG

Expression

Mammalian

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

Plasmid

#229811

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA transactivator-LexA-Operon-nls-GFP

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

Plasmid

#229819

Purpose

A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

Plasmid

#229831

Purpose

A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

Plasmid

#229837

Purpose

A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

Plasmid

#229838

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp

Use

Tol2 based expression vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-FL_WobbleMut

Plasmid

#209053

Purpose

Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8_WobbleMut

Plasmid

#209054

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8

Plasmid

#209048

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)

Plasmid

#156412

Purpose

Gateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancer

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

murine Dll4 F2- ETS site B/ site #2, 8X

Use

Gateway cloning

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA

Plasmid

#121804

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA

Plasmid

#121808

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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